Enzymes able to degrade or modify acyl-homoserine lactones (AHLs) have drawn considerable interest for their ability to interfere with the bacterial communication process referred to as quorum sensing. Many proteobacteria use AHL to coordinate virulence and biofilm formation in a cell density–dependent manner; thus, AHL-interfering enzymes constitute new promising antimicrobial candidates. Among these, lactonases and acylases have been particularly studied. These enzymes have been isolated from various bacterial, archaeal, or eukaryotic organisms and have been evaluated for their ability to control several pathogens. Engineering studies on these enzymes were carried out and successfully modulated their capacity to interact with specific AHL, increase their catalytic activity and stability, or enhance their biotechnological potential. In this review, special attention is paid to the screening, engineering, and applications of AHL-modifying enzymes. Prospects and future opportunities are also discussed with a view to developing potent candidates for bacterial control.

中文翻译：

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对细菌控制工程化酰基高丝氨酸内酯干扰酶。

能够降解或修饰酰基高丝氨酸内酯（AHL）的酶引起了人们极大的兴趣，因为它们具有干扰细菌交流过程（称为群体感应）的能力。许多蛋白细菌使用AHL以依赖细胞密度的方式协调毒力和生物膜的形成。因此，AHL干扰酶构成了新的有希望的抗微生物候选物。其中，已对乳糖酶和酰化酶进行了专门研究。这些酶已从各种细菌，古细菌或真核生物中分离出来，并已评估了它们控制几种病原体的能力。对这些酶进行了工程研究，并成功地调节了它们与特定AHL相互作用，增加其催化活性和稳定性或增强其生物技术潜力的能力。在这篇评论中，要特别注意AHL修饰酶的筛选，工程设计和应用。还讨论了前景和未来机会，以期开发出有效的细菌控制候选物。