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PAX7 promotes CD49f-positive dairy goat spermatogonial stem cells' self-renewal.
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2020-07-21 , DOI: 10.1002/jcp.29954 Xiaomin Du 1 , Siyu Wu 1 , Yudong Wei 1 , Xiuwei Yu 1 , Fanglin Ma 1 , Yuanxin Zhai 1 , Donghui Yang 1 , Mengfei Zhang 1 , Wenqing Liu 1 , Haijing Zhu 1, 2 , Jiang Wu 1, 3 , Mingzhi Liao 4 , Na Li 1 , Chunling Bai 5 , Guangpeng Li 5 , Jinlian Hua 1
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2020-07-21 , DOI: 10.1002/jcp.29954 Xiaomin Du 1 , Siyu Wu 1 , Yudong Wei 1 , Xiuwei Yu 1 , Fanglin Ma 1 , Yuanxin Zhai 1 , Donghui Yang 1 , Mengfei Zhang 1 , Wenqing Liu 1 , Haijing Zhu 1, 2 , Jiang Wu 1, 3 , Mingzhi Liao 4 , Na Li 1 , Chunling Bai 5 , Guangpeng Li 5 , Jinlian Hua 1
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Spermatogenesis is a complex process that originates from and depends on the spermatogonial stem cells (SSCs). The number of SSCs is rare, which makes the separation and enrichment of SSCs difficult and inefficient. The transcription factor PAX7 maintains fertility in normal spermatogenesis in mice. However, for large animals, much less is known about the SSCs' self‐renewal regulation, especially in dairy goats. We isolated and enriched the CD49f‐positive and negative dairy goat testicular cells by magnetic‐activated cell sorting strategies. The RNA‐ sequencing and experimental data revealed that cells with a high CD49f and PAX7 expression are undifferentiated spermatogonia in goat testis. Our findings indicated that ZBTB16 (PLZF), PAX7, LIN28A, BMPR1B, FGFR1, and FOXO1 were expressed higher in CD49f‐positive cells as compared to negative cells and goat fibroblasts cells. The expression and distribution of PAX7 in dairy goat also have been detected, which gradually decreased in testis tissue along with the increasing age. When the PAX7 gene was overexpressed in dairy goat immortal mGSCs‐I‐SB germ cell lines, the expression of PLZF, GFRα1, ID4, and OCT4 was upregulated. Together, our data demonstrated that there is a subset of spermatogonial stem cells with a high expression of PAX7 among the CD49f+ spermatogonia, and PAX7 can maintain the self‐renewal of CD49f‐positive SSCs.
中文翻译：
PAX7 促进 CD49f 阳性奶山羊精原干细胞的自我更新。
精子发生是一个复杂的过程，它起源于并依赖于精原干细胞 (SSC)。SSCs的数量稀少，这使得SSCs的分离和富集困难且效率低下。转录因子 PAX7 维持小鼠正常精子发生的生育能力。然而，对于大型动物，SSCs 的自我更新调节知之甚少，尤其是在奶山羊中。我们通过磁激活细胞分选策略分离并富集了 CD49f 阳性和阴性奶山羊睾丸细胞。RNA测序和实验数据表明，CD49f和PAX7高表达的细胞是山羊睾丸中未分化的精原细胞。我们的研究结果表明，ZBTB16 (PLZF)、PAX7、LIN28A、与阴性细胞和山羊成纤维细胞相比，BMPR1B、FGFR1和FOXO1在 CD49f 阳性细胞中表达更高。PAX7在奶山羊中的表达和分布也被检测到，随着年龄的增加，其在睾丸组织中的表达和分布逐渐减少。当 PAX7基因在奶山羊永生 mGSCs-I-SB 生殖细胞系中过表达时，PLZF、GFRα1、ID4 和 OCT4 的表达上调。总之，我们的数据表明，在 CD49f +精原细胞中有一个PAX7高表达的精原干细胞亚群，并且PAX7可以维持 CD49f 阳性 SSC 的自我更新。
更新日期：2020-07-21
中文翻译：
PAX7 促进 CD49f 阳性奶山羊精原干细胞的自我更新。
精子发生是一个复杂的过程，它起源于并依赖于精原干细胞 (SSC)。SSCs的数量稀少，这使得SSCs的分离和富集困难且效率低下。转录因子 PAX7 维持小鼠正常精子发生的生育能力。然而，对于大型动物，SSCs 的自我更新调节知之甚少，尤其是在奶山羊中。我们通过磁激活细胞分选策略分离并富集了 CD49f 阳性和阴性奶山羊睾丸细胞。RNA测序和实验数据表明，CD49f和PAX7高表达的细胞是山羊睾丸中未分化的精原细胞。我们的研究结果表明，ZBTB16 (PLZF)、PAX7、LIN28A、与阴性细胞和山羊成纤维细胞相比，BMPR1B、FGFR1和FOXO1在 CD49f 阳性细胞中表达更高。PAX7在奶山羊中的表达和分布也被检测到，随着年龄的增加，其在睾丸组织中的表达和分布逐渐减少。当 PAX7基因在奶山羊永生 mGSCs-I-SB 生殖细胞系中过表达时，PLZF、GFRα1、ID4 和 OCT4 的表达上调。总之，我们的数据表明，在 CD49f +精原细胞中有一个PAX7高表达的精原干细胞亚群，并且PAX7可以维持 CD49f 阳性 SSC 的自我更新。
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