Surface Immobilized Nucleic Acid-Transcription Factor Quantum Dots for Biosensing.,Advanced Healthcare Materials

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Surface Immobilized Nucleic Acid-Transcription Factor Quantum Dots for Biosensing.
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2020-07-21 , DOI: 10.1002/adhm.202000403
Mingfu Chen ₁ , Thuy T Nguyen ₁ , Nitinun Varongchayakul ₁ , Chloé Grazon _{2,

3} , Margaret Chern ₄ , R C Baer ₅ , Sébastien Lecommandoux ₃ , Catherine M Klapperich _{1,

4} , James E Galagan _{1,

5} , Allison M Dennis _{1,

4} , Mark W Grinstaff _{1,

2,

4}

Affiliation

Immobilization of biosensors on surfaces is a key step toward development of devices for real‐world applications. Here the preparation, characterization, and evaluation of a surface‐bound transcription factor–nucleic acid complex for analyte detection as an alternative to conventional systems employing aptamers or antibodies are described. The sensor consists of a gold surface modified with thiolated Cy5 fluorophore‐labeled DNA and an allosteric transcription factor (TetR) linked to a quantum dot (QD). Upon addition of anhydrotetracycline (aTc)—the analyte—the TetR‐QDs release from the surface‐bound DNA, resulting in loss of the Förster resonance energy transfer signal. The sensor responds in a dose‐dependent manner over the relevant range of 0–200 µm aTc with a limit of detection of 80 nm. The fabrication of the sensor and the subsequent real‐time quantitative measurements establish a framework for the design of future surface‐bound, affinity‐based biosensors using allosteric transcription factors for molecular recognition.

中文翻译：

用于生物传感的表面固定的核酸转录因子量子点。

将生物传感器固定在表面上是开发用于实际应用的设备的关键一步。这里介绍了一种表面结合的转录因子-核酸复合物的制备，表征和评估，用于分析物检测，作为使用适体或抗体的常规系统的替代方法。该传感器由经硫醇化Cy5荧光团标记的DNA修饰的金表面和与量子点（QD）连接的变构转录因子（TetR）组成。加入分析物脱水四环素（aTc）后，TetR-QDs从表面结合的DNA中释放出来，导致Förster共振能量转移信号丢失。传感器在0–200 µ m aTc的相关范围内以剂量依赖性方式响应，检测极限为80 n m。传感器的制造以及随后的实时定量测量为使用变构转录因子进行分子识别的未来基于表面亲和力的生物传感器的设计建立了框架。

更新日期：2020-09-10

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