Abstract Hop (Humulus lupulus) is highly susceptible to powdery mildew caused by the fungal pathogen Podosphaera macularis. The putative resistance (R) gene HCH1 is purported to encode an endochitinase with antifungal activity. Whilst expression of HCH1 is associated with fungal resistance, no studies to date have verified that HCH1 encodes an endochitinase. Herein bioinformatics approach is used to characterise the putative HCH1 gene and confirm the identity of the associated encoded protein: a family 19 class IB endochitinase. The characterisation of HCH1 provides insights into the evolved biotic stress response by hop, and thereby presents as a promising target for selective breeding of powdery mildew resistant crops.

中文翻译：

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推测的啤酒花抗真菌几丁质酶 HCH1 的生物信息学分析

摘要 啤酒花 (Humulus lupulus) 对真菌病原体 Podosphaera macularis 引起的白粉病高度敏感。假定的抗性 (R) 基因 HCH1 据称编码具有抗真菌活性的内切壳多糖酶。虽然 HCH1 的表达与真菌抗性有关，但迄今为止没有研究证实 HCH1 编码内切壳多糖酶。在本文中，生物信息学方法用于表征假定的 HCH1 基因并确认相关编码蛋白的身份：家族 19 类 IB 内切壳多糖酶。HCH1 的表征提供了对啤酒花进化生物胁迫反应的见解，从而成为选择性育种抗白粉病作物的有希望的目标。