In this study, the anti-obesity effect and mechanism of action of Viburnim opulus fruit crude extract (CE) and semi-purified phenolic extract (SPPE) in 3T3-L1 cells were studied. In addition, inhibitory activity of extracts against pancreatic lipase in triolein emulsion was estimated, as well as their phenolic profiles by UPLC/Q-TOF-MS. Twenty four phenolic compounds were identified in both extracts with chlorogenic acid as the dominant compound. The inhibition mode of extracts against lipase was competitive and SPPE demonstrated synergistic action with orlistat. Both extracts had the ability to inhibit adipogenesis process in a dose-dependent manner. Cells treatment with extracts at 75 µg/mL concentration was not only sufficient to inhibit lipids accumulation at almost 20%, but also influenced the size of lipid droplets. CE and SPPE reduced intracellular reactive oxygen species production by 20% and leptin level by 21–30%. They did not affect the peroxisome proliferator-activated receptor-gamma and adiponectin protein expression levels.

本研究探讨了荚burn的抗肥胖作用及其作用机理研究了3T3-L1细胞中的水果粗提取物（CE）和半纯化酚提取物（SPPE）。此外，通过UPLC / Q-TOF-MS评估了提取物对甘油三油精乳液中胰脂肪酶的抑制活性及其酚类特征。在两种提取物中均鉴定出二十四种酚类化合物，其中绿原酸为主要化合物。提取物对脂肪酶的抑制模式具有竞争性，SPPE与奥利司他具有协同作用。两种提取物均具有以剂量依赖性方式抑制脂肪形成过程的能力。用浓度为75 µg / mL的提取物处理细胞不仅足以抑制几乎20％的脂质积聚，而且还影响了脂质滴的大小。CE和SPPE可将细胞内活性氧的产生减少20％，将瘦素水平减少21–30％。