Leptotrombidium pallidum is the major vector mite for Orientia tsutsugamushi, the causative agent of scrub typhus, in Asian countries, including Korea. Despite its medical importance, L. pallidum has little genetic information available to date. To analyze the L. pallidum genome, we extracted genomic DNA (gDNA) from a single female of a 7-generation inbred L. pallidum colony and amplified the gDNA by whole genome amplification (WGA). The resulting amplified gDNA was used to construct paired-end and mate-pair libraries that were sequenced using Illumina platforms (HiSeq2000 and MiSeq). More than 45 Gb of sequence reads from both paired-end and mate-pair libraries of the WGA gDNA were trimmed and then de novo assembled using CLC Assembly Cell v.4.0 for contig assembly and SSPACE for scaffolding. The assembly generated approximately 6,545 scaffolds with an N50 value of 92,945 and total size of ~ 193 Mb. For gene predictions, the PASA and GeneWise models were used, and ab initio gene predictions were performed independently, resulting in the prediction of 15,842 genes. RNA-Seq expression profiles revealed constitutive expression of 11,572 unique protein-coding genes in larva, 12,364 in protonymph, 12,872 in male adult, and 12,617 in female adult stages. Of the 15,842 predicted genes, 10,885 were commonly expressed through all L. pallidum stages. Genes selectively over-transcribed in the larval stage, which is when host parasitization and disease transmission occur, were further annotated, and their putative roles were discussed.

在包括韩国在内的亚洲国家中，苍白细rom是bid虫病的东方病原体，是东方typ虫的主要媒介螨。尽管有医学上的重要性，但迄今为止，苍白乳杆菌尚无可用的遗传信息。为了分析苍白乳球菌的基因组，我们从7代自交苍白乳杆菌菌落的一位雌性中提取了基因组DNA（gDNA），并通过全基因组扩增（WGA）扩增了gDNA。所得扩增的gDNA用于构建配对末端和伴侣配对文库，并使用Illumina平台（HiSeq2000和MiSeq）进行测序。从WGA gDNA的配对末端和配对配对文库中读取的超过45 Gb的序列读段被修剪，然后从头开始使用CLC Assembly Cell v.4.0进行重叠群组装，使用SSPACE进行脚手架组装。组装产生了约6,545个支架，N50值为92,945，总大小约为193 Mb。对于基因预测，使用了PASA和GeneWise模型，并且独立进行了从头算基因的预测，从而预测了15,842个基因。RNA-Seq表达谱揭示了在幼虫中11,572个独特的蛋白质编码基因，在质子虫中的12,364，在雄性成年中的12,872和在成年雌性中的11,617的组成性表达。在15,842个预测基因中，有10,885个在所有苍白乳杆菌中普遍表达阶段。进一步注释了在幼虫阶段选择性地过度转录的基因，此时宿主发生寄生虫和疾病传播，并讨论了它们的假定作用。