The objective of this study was to research the effect of the freeze-drying process on the metabolic changes of Pseudomonas putida strains (E41, E42, R85) isolated from the interior of Sida hermaphrodita roots with the use of the phenotypic microarrays (PM) technology. The proposed method of the freeze-drying process with inulin as component lycoprotectant demonstrated a high bacterial survival ratio (BSR) immediately after freeze-drying and storage after 12 months. While, after 360 days of freeze-drying BSR decreased to value of 74.38. Pseudomonas putida strains were assayed on microplates PM1-PM5, and PM9-PM13 testing 664 different substrates. However, no significant differences in the use of C substrates were observed either before or after the freeze drying process. An insignificant negative effect of the freeze-drying on the use of these substrates was observed. The utilization of N, P and S sources was low or showed no metabolic activity for most of the compounds after freeze-drying. The freeze-drying process increased the sensitivity of the bacteria to antibiotics and selected chemicals. In this study, the freeze-drying process decreased the metabolic activities of the tested strains and their resistance to antibiotics and chemicals.

中文翻译：

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冷冻干燥过程对从雌雄同株健康根内部分离的恶臭假单胞菌菌株表型多样性的影响：表型微阵列（PMs）

本研究的目的是利用表型微阵列 (PM) 技术研究冷冻干燥过程对从雌雄同株根部内部分离的恶臭假单胞菌菌株 (E41、E42、R85) 代谢变化的影响。 . 所提出的以菊粉为成分的冻干保护剂的冷冻干燥工艺方法在冷冻干燥和储存 12 个月后立即显示出高细菌存活率 (BSR)。而冷冻干燥 360 天后，BSR 值下降到 74.38。在微孔板 PM1-PM5 和 PM9-PM13 上检测恶臭假单胞菌菌株，测试 664 种不同的底物。然而，在冷冻干燥过程之前或之后，没有观察到 C 底物的使用有显着差异。观察到冷冻干燥对这些底物的使用的微不足道的负面影响。冷冻干燥后，大多数化合物的 N、P 和 S 源利用率低或没有代谢活性。冷冻干燥过程增加了细菌对抗生素和特定化学品的敏感性。在这项研究中，冷冻干燥过程降低了受试菌株的代谢活动及其对抗生素和化学品的抵抗力。