Abstract Resveratrol is an antitoxin secreted by plants such as Polygonum cuspidatum Sieb. et Zucc and Vitis vinifera L. when they are attacked by pathogens. In the present study, three methods were used to prepare biological samples, and then an efficient strategy based on ultra-high-performance liquid chromatography-linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS) was developed to screen and identify resveratrol metabolites in rat urine, plasma and faeces. As a result, a total of 56 resveratrol metabolites were screened and characterized. Among them, 39 metabolites were found in rat urine, while 6 and 16 metabolites were characterized from rat plasma and faeces, respectively. In addition, 56, 12 and 15 metabolic products were screened by solid phase extraction (method I), methanol precipitation (method II) and acetonitrile precipitation (method III), respectively, indicating that method I could be adopted as the most acceptable method. The results also demonstrated that resveratrol mainly underwent glucuronidation, glucosylation, sulfation, hydroxylation, dehydrogenation, hydrogenation, methylation and their composite reactions. Moreover, these metabolic reactions occurred to form a possible metabolic network that is similar to a triangular pyramid model. In summary, this research provides an idea for the further study of drug metabolism.

中文翻译：

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超高效液相色谱-高分辨质谱综合分析大鼠体内白藜芦醇代谢物

摘要 白藜芦醇是虎杖等植物分泌的一种抗毒素。当 Zucc 和 Vitis vinifera L. 受到病原体攻击时。在本研究中，采用三种方法制备生物样品，然后开发了一种基于超高效液相色谱-线性离子阱-Orbitrap 质谱（UHPLC-LTQ-Orbitrap MS）的有效策略来筛选和鉴定大鼠尿液、血浆和粪便中的白藜芦醇代谢物。结果，共筛选和表征了 56 种白藜芦醇代谢物。其中，在大鼠尿液中发现了 39 种代谢物，而分别从大鼠血浆和粪便中发现了 6 种和 16 种代谢物。此外，通过固相萃取（方法 I）筛选了 56、12 和 15 种代谢产物，分别为甲醇沉淀（方法Ⅱ）和乙腈沉淀（方法Ⅲ），表明方法Ⅰ可以作为最可接受的方法。结果还表明，白藜芦醇主要发生葡萄糖醛酸化、糖基化、硫酸化、羟基化、脱氢、氢化、甲基化及其复合反应。此外，这些代谢反应的发生形成了一个可能的代谢网络，类似于三角金字塔模型。综上所述，本研究为进一步研究药物代谢提供了思路。甲基化及其复合反应。此外，这些代谢反应的发生形成了一个可能的代谢网络，类似于三角金字塔模型。综上所述，本研究为进一步研究药物代谢提供了思路。甲基化及其复合反应。此外，这些代谢反应的发生形成了一个可能的代谢网络，类似于三角金字塔模型。综上所述，本研究为进一步研究药物代谢提供了思路。