Anaerobic bioremediation of tetrachloroethylene (PCE) under high concentration conditions is difficult. Anaerobic dechlorination of PCE occurs with synergetic reactions, fermentation, and sulfate-reduction; however, the way in which high concentrations of PCE affects these reactions is still poorly understood. This study aims to elucidate how high concentrations of PCE affect fermentation and sulfate-reduction, as well as PCE dechlorination. Laboratory dechlorination tests were performed using a wide concentration range of PCE between 2 and 125 mg/L added to a microbial consortium that had been continuously cultivated in the laboratory and completely dechlorinated PCE for over 4 years. Fermentation of lactate, reduction of sulfate, and dechlorination of PCE were monitored in addition to microbial activities based on RNA. All three reactions, fermentation, sulfate-reduction, and PCE dechlorination were observed to be inhibited. The inhibition for fermentation, sulfate-reduction, and dechlorination occurred when PCE concentrations were higher than 125, 75, and 30 mg/L, respectively. The fermenter, Anaerotignum, and the sulfate-reducer, Desulfosporosinus, were active when the dechlorination was inhibited with 30 mg/L of PCE. These findings suggest that there is interference of PCE dechlorination, despite the occurrence of fermentation and sulfate reduction. Bioaugmentation with a PCE dechlorinator that is tolerant to high PCE concentrations can be a possible solution for bioremediation of PCE when its concentrations are greater than 30 mg/L.

在高浓度条件下对四氯乙烯（PCE）进行厌氧生物修复是困难的。PCE的厌氧脱氯作用是协同反应，发酵和硫酸盐还原作用。但是，对于高浓度PCE影响这些反应的方式仍然知之甚少。这项研究旨在阐明高浓度的PCE如何影响发酵和硫酸盐还原以及PCE脱氯。实验室脱氯测试是通过将浓度范围在2至125 mg / L的PCE广泛添加到已在实验室中连续培养并完全脱氯4年以上的微生物协会中进行的。除了基于RNA的微生物活性外，还监测了乳酸的发酵，硫酸盐的还原和PCE的脱氯。这三个反应 观察到发酵，硫酸盐还原和PCE脱氯被抑制。当PCE浓度分别高于125、75和30 mg / L时，发生了对发酵，硫酸盐还原和脱氯的抑制作用。发酵罐Anaerotignum和硫酸盐还原，Desulfosporosinus，活跃时脱氯用30毫克/升的PCE抑制。这些发现表明，尽管发生了发酵和硫酸盐还原，但仍存在PCE脱氯的干扰。当PCE浓度大于30 mg / L时，用PCE脱氯剂对PCE的高浓度进行生物强化可能是PCE生物修复的一种可能的解决方案。