Four Dendrobium Sonia ‘Earsakul’ lines were generated by insertion of one, two or three antisense copies of a Carica papaya gene encoding 1-aminocyclopropane-1-carboxylic acid oxidase (CpACO). Whole vegetative plants of the transgenic lines showed about 50% of the basal ethylene production rate, while the increase in ethylene production in floral buds during opening and open flowers prior to visible senescence was delayed. Detailed analysis of more than 100 parameters in flowering plants showed no effect of antisense ACO on plant morphology and coloration, except for shorter length and width of some of the sepals and petals. In intact plants the water-soaking of floral buds as well as bud abscission were delayed by ACO antisense, as was the time to senescence of open flowers. Pollen viability and pollen tube growth were not affected in the transgenic lines. In cut inflorescences placed in water, bud yellowing, bud water soaking, and bud abscission were considerably delayed by the antisense construct, while the life span of open flowers were increased and abscission of open flowers were delayed. It is concluded that the reduction of ACO activity affected the shape of some petals/sepals and delayed the abortion in floral buds, and the senescence and abscission of open flowers.

四石斛通过的中的一个，两个或三个反义拷贝插入产生了Sonia“Earsakul”线番木瓜编码1-氨基环丙烷-1-羧酸氧化酶（基因CpACO）。转基因品系的全营养植物显示出约50％的基础乙烯产生率，而花蕾在可见衰老之前的开放和开放花期乙烯产量的增加被延迟。对开花植物中100多个参数的详细分析显示，反义ACO对植物形态和着色没有影响，除了某些萼片和花瓣的长度和宽度较短。在完整的植物中，ACO延迟了花蕾的浸水和芽脱落反义，如开放花衰老的时间。在转基因品系中，花粉活力和花粉管生长不受影响。在切花序中，通过反义构建体可显着延迟花蕾泛黄，花蕾浸水和芽脱落，而延长开放花的寿命并延迟开放花的脱落。结论是ACO活性的降低影响了一些花瓣/花萼的形状并延迟了花蕾的流产，以及开放花的衰老和脱落。