The importance of tumour microenvironment in tumour behaviour has now become clearer. This study aimed to determine the prognostic effect of the proportion of tumour-stroma (PTS) in metastatic lymph nodes of advanced-stage colon cancers (CCs). We investigated PTS in positive lymph nodes of stage III-IV CC patients who underwent surgical treatment between 2004 and 2014. We used a standard approach in methodology. PTS was significantly associated with prognostic factors in the metastatic lymph nodes (perineural invasion [p = 0.031], lymphatic invasion [p = 0.032], invasive margin [p = 0.043], advanced pT [p = 0.020], and margin involvement [p = 0.034]). In addition, the correlations between PTS estimates (R = 0.704 to 0.617, p < 0.001), the reproducibility of the research (Κappa = 0.72–0.68) and the usefulness of the cut-off value (ROC: 50.33%; AUC = 0.752 [0.667–0.857]) were successful. In univariate analysis, 5-year survival was poor for RFS (p < 0.001), OS (p = 0.001) and LR (p = 0.013) in high PTS patients. Multivariate analysis confirmed that high PTS was an independent worse parameter for RFS (HR = 1.32, 95% CI: 1.17–2.55, p = 0.001) and OS (HR = 1.37, 95% CI: 1.25–1 - 2.56, p = 0.009). In this study, we showed that high PTS in metastatic lymph nodes was a successful prognostic marker for advanced-stage CCs. Also, the standard approach we used for the methodology was successful.

肿瘤微环境在肿瘤行为中的重要性现已变得更加清楚。本研究旨在确定晚期结肠癌（CC）转移淋巴结中肿瘤基质（PTS）比例的预后作用。我们调查了 2004 年至 2014 年间接受手术治疗的 III-IV 期 CC 患者的阳性淋巴结中的 PTS。我们在方法学上使用了标准方法。 PTS 与转移淋巴结的预后因素显着相关（神经周围浸润 [ p  = 0.031]、淋巴管浸润 [ p  = 0.032]、浸润边缘 [ p  = 0.043]、晚期 pT [ p  = 0.020] 和边缘受累 [ p  = 0.034]）。此外，PTS 估计值（R = 0.704 至 0.617，p  < 0.001）、研究的再现性（Κappa = 0.72–0.68）和截止值的有​​用性（ROC：50.33%；AUC = 0.752）之间的相关性[0.667–0.857]）是成功的。在单变量分析中， 高 PTS 患者的RFS ( p  < 0.001)、OS ( p  = 0.001) 和 LR ( p = 0.013)的 5 年生存率较差。多变量分析证实，高 PTS 是 RFS（HR = 1.32，95% CI：1.17–2.55， p  = 0.001）和 OS（HR = 1.37，95% CI：1.25–1 - 2.56，p  = 0.009 ）的独立较差参数）。在这项研究中，我们证明转移淋巴结中的高 PTS 是晚期 CC 的成功预后标志。此外，我们用于该方法的标准方法也是成功的。