Intracerebroventricular (ICV) injection of streptozotocin (STZ) is a well established procedure to induce neuroinflammation leading to dementia in experimental animals. However, the optimal dose of STZ has not been determined. In the present study, rats were ICV injected with 1.5, 3 and 6 mg of STZ per kg of body weight. After 21 days, neuroinflammatory markers i.e. TNF-α, IL-1β, ROS and nitrite were quantified in the hippocampus. Memory function was assessed by the radial arm maze test after 9, 12, 15, 18, 21 days following STZ injection. STZ treatment significantly increased neuroinflammatory markers and decreased memory functions in a dose dependent manner showing optimum effects at the dose of 3 mg/kg.

脑室内 (ICV) 注射链脲佐菌素 (STZ) 是一种成熟的程序，可诱导神经炎症导致实验动物痴呆。然而，STZ 的最佳剂量尚未确定。在本研究中，大鼠被 ICV 注射了 1.5、3 和 6 毫克每公斤体重的 STZ。21 天后，在海马中量化神经炎症标志物，即 TNF-α、IL-1β、ROS 和亚硝酸盐。在注射 STZ 后 9、12、15、18、21 天后，通过桡臂迷宫测试评估记忆功能。STZ 治疗以剂量依赖性方式显着增加神经炎症标志物并降低记忆功能，在 3 mg/kg 剂量下显示最佳效果。