Purpose

Sporothrix schenckii is a thermally dimorphic fungus. In a saprotrophic environment or culturing at 25 °C, it grows as mycelia, whereas in host tissues or culturing at 37 °C, it undergoes dimorphic transition and division into pathogenic yeast cells. S. schenckii can cause serious disseminated sporotrichosis in immunocompromised hosts and presents an emerging global health problem. The mycelium-to-yeast transition was a consequence of the adaptive process to different environment. Some studies showed that the transition was significantly related to the virulence and pathogenesis of dimorphic fungi. However the genetic mechanisms of this complicated biological process are poorly understood.

Method

Our study presented a comparative transcriptomic analysis perspective on temperature stress in a visceral isolates of S. schenckii, obtaining more genetic information related to dimorphic transition.

Results

The 9.38 Gbp dataset was generated and assembled into 14,423 unigenes. Compared with gene and protein databases, 9561 unigenes were annotated. Comparative analysis identified 1259 genes expressed differentially in mycelium and yeast phase, and were categorized into a number of important biological processes, such as synthesis and metabolism, transmembrane transport, biocatalysis, oxidation reduction, and cellular signal transduction.

Conclusions

The findings suggested that temperature-dependent transition was tightly associated with stress adaptation, growth and development, signal regulation, adhesion, and colonization, which was predicted to be related with virulence and pathogenesis. Collection of these data should offer fine-scale insights into the mechanisms of dimorphism and pathogenesis of S. schenckii, and meanwhile facilitate the evolutionary and function studies of other dimorphic fungi.

中文翻译：

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暴露于温度胁迫下的双相真菌申克孢子丝菌的转录组分析。

目的

申克孢子丝菌是一种热二态性真菌。在腐生环境或25°C培养时，它以菌丝体的形式生长，而在宿主组织中或在37°C培养时，它经历二态性转变并分裂成致病性酵母细胞。S. schenckii可在免疫功能低下的宿主中引起严重的播散性孢子丝菌病，并成为一个新出现的全球健康问题。菌丝体到酵母的转变是对不同环境的适应过程的结果。一些研究表明，这种转变与二态性真菌的毒力和发病机制显着相关。然而，人们对这一复杂生物过程的遗传机制知之甚少。

方法

我们的研究提出了关于申克内脏分离株中温度胁迫的比较转录组学分析观点，获得了更多与双态转变相关的遗传信息。

结果

生成 9.38 Gbp 数据集并组装成 14,423 个 unigenes。与基因和蛋白质数据库相比，9561个unigenes被注释。比较分析确定了1259个基因在菌丝体和酵母期差异表达，并被归类为许多重要的生物过程，如合成和代谢、跨膜转运、生物催化、氧化还原和细胞信号转导。

结论

研究结果表明，温度依赖性转变与应激适应、生长发育、信号调节、粘附和定植密切相关，预计这与毒力和发病机制有关。这些数据的收集将为申克双态性机制和发病机制提供更精细的见解，同时促进其他双态性真菌的进化和功能研究。