Electronic waste is an evolving source of harmful pollutants in our surrounding environments and considered to be perilous as it contains toxic metals such as chromium, cadmium, lead, mercury, zinc, and nickel in huge quantities. Heavy metals are harmful contaminants and accumulated in the environment due to various anthropogenic activities. The present study was conducted to isolate and characterize different heavy metal tolerant bacterial species, based on molecular techniques from soil contaminated by electronic waste. The contaminated soil samples were analyzed for various physicochemical properties such as pH, electrical conductivity, soil moisture, water holding capacity, organic carbon, organic matter, available phosphorus, total nitrogen, and potassium using standard procedures. The soil samples were found to contain a higher amount of different heavy metals such as copper, chromium, lead, iron, cadmium, and nickel. Serial dilution and spread plate techniques have been used for bacterial isolation. The identification and molecular characterization of isolated bacterial species were done by biochemical tests and 16S rRNA gene sequencing technique. The 16S rRNA sequencing analysis confirmed the presence of different bacterial species as, Micrococcus aloeverae, Kocuria turfanensis, Bacillus licheniformis, Bacillus jeotgali, Bacillus velezensis, and Bacillus haikouensis. The findings indicated that the e-waste dumping sites are the storehouse of elite bacterial species. The present research study offers a platform for systematic analysis of e-waste sites by microbial profiling that may help in the innovation of novel microorganisms of scientific importance and better biotechnological potential.

电子废物是周围环境中不断发展的有害污染物的源头，并且由于含有大量的铬，镉，铅，汞，锌和镍等有毒金属，因此被认为是危险的。重金属是有害的污染物，由于各种人为活动在环境中积累。本研究是基于分子技术从受电子废物污染的土壤中分离并表征不同的重金属耐受细菌种类的。使用标准程序分析受污染的土壤样品的各种物理化学特性，例如pH，电导率，土壤湿度，持水量，有机碳，有机质，有效磷，总氮和钾。发现土壤样品中含有大量不同的重金属，例如铜，铬，铅，铁，镉和镍。连续稀释和铺板技术已经用于细菌分离。通过生化测试和16S rRNA基因测序技术对分离出的细菌进行鉴定和分子表征。16S rRNA测序分析证实存在不同的细菌，因为微小微球菌（Alcoevercus aloeverae），臭椿（Kocuria turfanensis），地衣芽孢杆菌（Bacillus licheniformis），芽孢杆菌（芽孢杆菌），芽孢杆菌（Bacillus velezensis）和海口芽孢杆菌（Haikouensis）。调查结果表明，电子废物倾倒场所是精英细菌物种的仓库。本研究提供了一个平台，可通过微生物谱系统分析电子废物场所，这可能有助于创新具有科学重要性和更好生物技术潜力的新型微生物。