Angiotensin I converting enzyme (ACE) inhibition is one of the key factors for cardiovascular disease, and inhibition of ACE activity is related to the prevention of high blood pressure. Until now, some ACE inhibitory peptides have been found in the protein hydrolysates of red algae, and most of them were derived from phycobiliproteins and rubisco. In this study, we modified the preparation method to evaluate the potential of ACE inhibitory activity from protein hydrolysate of red alga Mazzaella japonica except for phycobiliproteins and rubisco. As a result, we identified 11 peptides (YRD, VSEGLD, TIMPHPR, GGPAT, SSNDYPI, SRIYNVKSNG, VDAHY, CPYDWV, YGDPDHY, NLGN and DFGVPGHEP) in the hydrolysate by the reversed phase-HPLC and MALDI-TOF/MS/MS. Among them, YRD would be derived from phycoerythrin α-subunit. However, the others would be from other proteins encoded in chloroplast or nuclear genomes. This study revealed that not only phycobiliproteins and rubisco but also the other proteins in red algae have the potential for the source of ACE inhibitory peptide.

血管紧张素I转换酶（ACE）的抑制是心血管疾病的关键因素之一，而抑制ACE活性与预防高血压有关。迄今为止，在红藻的蛋白水解物中已经发现了一些ACE抑制肽，其中大多数都来自藻胆蛋白和rubisco。在这项研究中，我们修改了制备方法，以评估红藻马扎氏菌蛋白质水解产物中ACE抑制活性的潜力。除了藻胆蛋白和rubisco。结果，我们通过反相HPLC和MALDI-TOF / MS / MS确定了水解产物中的11种肽（YRD，VSEGLD，TIMPHPR，GGPAT，SSNDYPI，SRIYNVKSNG，VDAHY，CPYDWV，YGDPDHY，NLGN和DFGVPGHEP）。其中，YRD来源于藻红蛋白α-亚基。然而，其他将来自叶绿体或核基因组中编码的其他蛋白质。这项研究表明，不仅藻胆蛋白和rubisco，还有红藻中的其他蛋白都有可能是ACE抑制肽的来源。