With the aim of developing environment-friendly agricultural products with antifungal activity against Fusarium oxysporum f. sp. lycopersici, a causative agent of Fusarium wilt, active substances from Boesenbergia pulcherrima roots were isolated. The hexane fraction from B. pulcherrima root extract was analyzed by GC/MS. The main peaks were estimated and identified to be methyl eugenol, methyl isoeugenol, elemicin, α-asarone, and 1,2-dimethoxy-4-(2-methoxyethenyl)benzene based on the Wiley library and by comparing retention times and mass spectra with their corresponding standards using GC/MS. For the identification of the compound in peak D that was estimated to be 1,2,4-trimethoxy-5-vinylbenzene, for which no reference standard was available, the hexane fraction was processed by column chromatography before NMR analysis. The result confirmed the compound to be 1,2,4-trimethoxy-5-vinylbenzene. Almost all compounds showed antifungal activity against F. oxysporum based on bioassays, and α-asarone had the highest activity. Therefore, B. pulcherrima root extract can be a potential source of environment-friendly agricultural products with antifungal activity against F. oxysporum.

中文翻译：

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从提取的精油的抗真菌成分的标识提琴形凹唇一品红对枯萎病（尖镰孢）

为了开发对枯萎病菌具有抗真菌活性的环保农产品。sp。枯萎病的病原菌lycopersici，从波森勃氏根中分离出活性物质。通过GC / MS分析了来自B. pulcherrima根提取物的己烷部分。根据Wiley文库并通过比较保留时间和质谱图，估计并鉴定出主要峰为甲基丁香酚，甲基异丁香酚，elemicin，α-细辛醇和1,2-二甲氧基-4-（2-甲氧基乙烯基）苯。使用GC / MS的相应标准。为了鉴定峰D中估计为1,2,4-三甲氧基-5-乙烯基苯的化合物（无参考标准品），在进行NMR分析之前，通过柱色谱法处理己烷馏分。结果证实该化合物为1,2,4-三甲氧基-5-乙烯基苯。根据生物测定法，几乎所有化合物都对霉菌具有抗真菌活性，其中α-细辛醚活性最高。因此，B。pulcherrima根提取物可能是对农杆菌具有抗真菌活性的环保农产品的潜在来源。