A procedure has been developed for the determination of third-generation synthetic cannabinoids in oral fluid samples by using a semi-automated microextraction by packed sorbent (MEPS) procedure and gas chromatography–mass spectrometry (GC–MS) determination. Five synthetic cannabinoids were employed as model compounds 5F-ADB, MMB-CHMICA, THJ-2201, CUMYL-4CN-BINACA and MDMB-CHMCZCA. The most adequate operative conditions for MEPS were evaluated giving quantitative recoveries, from 89 to 124%, in synthetic and field saliva samples spiked with 125 and 250 μg/L of the studied cannabinoids, with the exception of MDMB-CHMCZCA in field saliva samples that provided slightly lower recoveries from 62 to 66%. A high sensitivity was obtained for the proposed MEPS-GC–MS procedure with limits of detection from 10 to 20 μg/L. The obtained results demonstrate the high potential of MEPS-GC–MS combination for semi-automated, selective and sensitive determination of synthetic cannabinoids in oral fluid samples.

中文翻译：

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口服液中第三代合成大麻素的测定

通过使用填充吸附剂（MEPS）的半自动微萃取和气相色谱-质谱（GC-MS）的测定，已经开发了一种测定口腔液样品中第三代合成大麻素的程序。五个合成大麻素被用作模型化合物5F-ADB，MMB-CHMICA，THJ-2201，CUMYL-4CN-BINACA和MDMB-CHMCZCA。在掺有125和250μg/ L所研究大麻素的合成唾液和田间唾液样品中，对MEPS的最合适的操作条件进行了评估，定量回收率从89％到124％，但田间唾液样品中的MDMB-CHMCZCA除外，回收率略低，从62％降至66％。拟议的MEPS-GC-MS方法获得了高灵敏度，检测限为10至20μg/ L。