New psychoactive substances (NPS) are a major public health problem, primarily due to the increased number of acute poisoning cases. Detection of these substances is a challenge. The aim of this research was to develop and validate a sensitive screening method for 104 drugs of abuse, including synthetic cannabinoids, synthetic cathinones, fentanyl analogues, phenethylamines and other abused psychoactive compounds (i.e., THC, MDMA, LSD and their metabolites) in oral fluid by liquid chromatography–tandem mass spectrometry (LC–MS-MS). The Quantisal™ oral fluid device was used to collect oral fluid samples. The oral fluid–elution buffer mixture (500-μL sample) was extracted with t-butyl methyl ether, and chromatographic separation was performed on a Raptor™ biphenyl column (100 × 2.1 mm ID, 2.7 μm), with a total run time of 13.5 min. Limits of detection were established at three concentrations (0.05, 0.1 or 1 ng/mL) for most analytes, except for acetyl norfentanyl and mescaline (5 ng/mL). Matrix effects were generally <20% and overall extraction recoveries >60%. The highest matrix effect was observed within the synthetic cannabinoid group (PB22, −55.5%). Lower recoveries were observed for 2C-T (47.2%) and JWH-175 (58.7%). Recoveries from the Quantisal™ device were also evaluated for all analytes (56.7–127%), with lower recoveries noted for 25I-NBOMe, valerylfentanyl and mCPP (56.7, 63.0 and 69.9%, respectively). Drug stability in oral fluid was evaluated at 15, 60 and 90 days and at 25, 4 and −20°C. As expected, greater stability was observed when samples were stored at −20°C, but even when frozen, some NPS (e.g., synthetic cannabinoids) showed more than 20% degradation. The method was successfully applied to the analysis of seven authentic oral fluid samples positive for 17 different analytes. The method achieved good sensitivity and simultaneous detection of a wide range of NPS.

中文翻译：

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通过LC-MS-MS筛查口腔液中104种新的精神活性物质（NPS）和其他滥用药物。

新的精神活性物质（NPS）是主要的公共卫生问题，主要是由于急性中毒病例的增加。这些物质的检测是一个挑战。这项研究的目的是开发和验证一种针对104种滥用药物的敏感筛选方法，包括合成大麻素，合成卡西酮，芬太尼类似物，苯乙胺和其他滥用的精神活性化合物（例如，THC，MDMA，LSD及其代谢物）液相色谱-串联质谱法（LC-MS-MS）分析液体。使用Quantisal™口腔液设备收集口腔液样品。用t提取口腔液-洗脱缓冲液混合物（500-μL样品）-丁基甲基醚，并在Raptor™联苯色谱柱（100×2.1 mm内径，2.7μm）上进行色谱分离，总运行时间为13.5分钟。除乙酰基去甲芬太尼和甲卡斯汀（5 ng / mL）外，大多数分析物的检测限设定为三种浓度（0.05、0.1或1 ng / mL）。基质效应通常<20％，总萃取回收率> 60％。在合成大麻素组中观察到最高的基质效应（PB22，-55.5％）。2C-T（47.2％）和JWH-175（58.7％）的回收率较低。还评估了Quantisal™设备的所有分析物的回收率（56.7–127％），其中25I-NBOMe，戊酰芬太尼和mCPP的回收率较低（分别为56.7、63.0和69.9％）。在15、60和90天以及25、4和-20°C下评估了口服液中的药物稳定性。不出所料 当样品在-20°C下保存时，观察到更大的稳定性，但是即使冷冻，一些NPS（例如合成大麻素）也显示出超过20％的降解。该方法已成功应用于分析17种不同分析物呈阳性的7种真实口腔液样品。该方法获得了良好的灵敏度并同时检测了多种NPS。