In this research, an aqueous extract of Daphne mucronata leaves with different salt sources including Zn(NO₃)₂ and ZnSO₄ solutions were used to synthesize ZnO-N and ZnO-S NPs, respectively. The physicochemical, antibacterial, antioxidant, and cytotoxic properties of these NPs were also surveyed. SEM and TEM images confirmed that the ZnO-N and ZnO-S NPs were polydispersed with spherical and plate shape and average diameters of 129 ± 45.49 nm and 87 ± 31.61 nm, respectively. The minimum bactericidal concentration (MBC) values for both NPs against bacterial strains were 2 mg/ml. The biofilm growth in biofilm producer strains was completely inhibited at minimum inhibitory concentration (MIC). The IC50 values for ZnO-N and ZnO-S NPs were calculated as 1.946 mg/ml and 0.369 mg/ml, respectively. Finally, due to the antioxidant, antimicrobial, and cytotoxic properties of zinc oxide NPs, green synthesis of ZnONPs using D. mucronata can improve their applications in a wide range of clinical areas.

在这项研究中，水蚤的水提取物具有不同的盐源，包括Zn（NO ₃）₂和ZnSO ₄溶液分别用于合成ZnO-N和ZnO-S NP。还调查了这些NP的理化，抗菌，抗氧化剂和细胞毒性。SEM和TEM图像证实ZnO-N和ZnO-S NPs呈球形和板状多分散，平均直径分别为129±45.49 nm和87±31.61 nm。两种NP对细菌菌株的最小杀菌浓度（MBC）值为2 mg / ml。在最小抑制浓度（MIC）下，生物膜生产菌株中的生物膜生长被完全抑制。ZnO-N和ZnO-S NP的IC50值分别计算为1.946 mg / ml和0.369 mg / ml。最后，由于氧化锌NP的抗氧化剂，抗微生物剂和细胞毒性，因此使用粘枝线虫（D. mucronata）绿色合成ZnONP的方法 可以改善其在广泛临床领域中的应用。