Abstract As an important factor affecting the structure of microbial communities, salinity has traditionally been a focus of exploratory studies of microorganisms. Here, we defined a salinity gradient with reference to the degree of natural salinization in a lake and withdrew the influence of irrelevant factors to explore the impacts of changes in salinity on the biological community. With the gradual increase in salinity, we found that Firmicutes replaced Bacteroidetes as the dominant flora. Through a network diagram, we found that Bacteroidetes had a weaker taxonomic correlation with the bacterial community in the saline environment, which indicated that the increase of salinity did play a role in filtering the bacterial community. In addition, we also found the typical freshwater bacteria GKS98 in a higher salinity environment, which supports the idea proposed by previous researchers that salinity creates a wider niche. However, our functional prediction results show that salinity is not the main factor affecting the functional characteristics of bacterial communities. Moreover, through a network diagram, we found that the symbiotic relationship between GKS98 and other strains also changed with increasing salinity. This suggests that the effects of salinity on microorganisms may involve additional ecological mechanisms such as niche occupation and functional interdependence within the community.

中文翻译：

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功能预测和网络分析研究微生物群落对单一环境因素的响应

摘要 作为影响微生物群落结构的重要因素，盐度历来是微生物探索性研究的重点。在这里，我们参考湖泊中的自然盐化程度定义了盐度梯度，并排除了无关因素的影响，以探索盐度变化对生物群落的影响。随着盐度的逐渐增加，我们发现厚壁菌门取代拟杆菌门成为优势菌群。通过网络图，我们发现拟杆菌与盐水环境中的细菌群落的分类相关性较弱，这表明盐度的增加确实起到了过滤细菌群落的作用。此外，我们还在较高盐度的环境中发现了典型的淡水细菌GKS98，这支持了先前研究人员提出的想法，即盐度创造了更广泛的生态位。然而，我们的功能预测结果表明，盐度不是影响细菌群落功能特征的主要因素。此外，通过网络图，我们发现 GKS98 与其他菌株之间的共生关系也随着盐度的增加而发生变化。这表明盐度对微生物的影响可能涉及额外的生态机制，例如群落内的生态位占据和功能相互依赖。我们发现 GKS98 与其他菌株之间的共生关系也随着盐度的增加而改变。这表明盐度对微生物的影响可能涉及额外的生态机制，例如群落内的生态位占据和功能相互依赖。我们发现 GKS98 与其他菌株之间的共生关系也随着盐度的增加而改变。这表明盐度对微生物的影响可能涉及额外的生态机制，例如群落内的生态位占据和功能相互依赖。