Abstract

A novel water-soluble polysaccharide, named ICP-1, was isolated and purified by Sephadex G-200 after extracting the crude polysaccharide (ICP) from Imperial Chrysanthemum. The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test. Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential. The results showed that ICP-1 was an acidic hetero-polysaccharide with an average molecular weight of 2.98 × 10³ kDa and a specific optical rotation of +155°. The glycosyl residues of ICP-1 were composed of (1→), (1→4) and (1→6) glucose, (1→5) arabinose, (1→4) galacturonic acid and (1→3,6) mannose. Besides, ICP-1 can speed up the acid production of lactic acid bacteria and promote the growth and proliferation of lactic acid bacteria effectively.

摘要

采用Sephadex G-200从帝王菊中提取粗多糖(ICP)后，分离纯化出一种新型水溶性多糖，命名为ICP-1 。ICP-1的结构表征通过物理和化学方法、FT-IR、NMR、SEM、HPGPC、高碘酸盐氧化、Smith降解、甲基化和刚果红试验确定。然后，测量乳酸菌的产酸和增殖以及模拟胃肠液的耐受性试验，以研究益生元潜力的活性。结果表明ICP-1是一种酸性杂多糖，平均分子量为2.98×10 ³ kDa 和 +155° 的特定旋光度。ICP-1的糖基残基由(1→)、(1→4)和(1→6)葡萄糖、(1→5)阿拉伯糖、(1→4)半乳糖醛酸和(1→3,6)组成甘露糖。此外，ICP-1可以加速乳酸菌的产酸，有效促进乳酸菌的生长和增殖。