Regulating the collective migration of cells is an important issue in bioengineering. Enhancing or suppressing cell migration and controlling the migration direction is useful for various physiological phenomena such as wound healing. Several methods of migration regulation based on different mechanical stimuli have been reported. While vibrational stimuli, such as sound waves, show promise for regulating migration, the effect of the vibration direction on collective cell migration has not been studied in depth. Therefore, we fabricated a vibrating system that can apply horizontal vibration to a cell culture dish. Here, we evaluated the effect of the vibration direction on the collective migration of fibroblasts in a wound model comprising two culture areas separated by a gap. Results showed that the vibration direction affects the cell migration distance: vibration orthogonal to the gap enhances the collective cell migration distance while vibration parallel to the gap suppresses it. Results also showed that conditions leading to enhanced migration distance were also associated with elevated glucose consumption. Furthermore, under conditions promoting cell migration, the cell nuclei become elongated and oriented orthogonal to the gap. In contrast, under conditions that reduce the migration distance, cell nuclei were oriented to the direction parallel to the gap.

中文翻译：

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成纤维细胞的集体细胞迁移受细胞培养皿水平振动的影响


调节细胞的集体迁移是生物工程中的一个重要问题。增强或抑制细胞迁移并控制迁移方向对于各种生理现象（例如伤口愈合）是有用的。已经报道了几种基于不同机械刺激的迁移调节方法。虽然声波等振动刺激有望调节迁移，但振动方向对集体细胞迁移的影响尚未得到深入研究。因此，我们制造了一个振动系统，可以对细胞培养皿施加水平振动。在这里，我们评估了伤口模型中振动方向对成纤维细胞集体迁移的影响，该伤口模型包含两个由间隙隔开的培养区域。结果表明，振动方向影响细胞迁移距离：垂直于间隙的振动增强了集体细胞迁移距离，而平行于间隙的振动抑制了它。结果还表明，导致迁移距离增加的条件也与葡萄糖消耗增加有关。此外，在促进细胞迁移的条件下，细胞核变得伸长并且定向为与间隙正交。相反，在减少迁移距离的条件下，细胞核定向于平行于间隙的方向。