Targeted suppression of soybean BAG6-induced cell death in yeast by soybean cyst nematode effectors.,Molecular Plant Pathology

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Targeted suppression of soybean BAG6-induced cell death in yeast by soybean cyst nematode effectors.
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2020-07-19 , DOI: 10.1111/mpp.12970
Jianying Wang ₁ , Greg Yeckel ₁ , Pramod K Kandoth ₁ , Lakmini Wasala ₁ , Richard S Hussey ₂ , Eric L Davis ₃ , Thomas J Baum ₄ , Melissa G Mitchum _{1,

5}

Affiliation

While numerous effectors that suppress plant immunity have been identified from bacteria, fungi, and oomycete pathogens, relatively little is known for nematode effectors. Several dozen effectors have been reported from the soybean cyst nematode (SCN). Previous studies suggest that a hypersensitive response‐like programmed cell death is triggered at nematode feeding sites in soybean during an incompatible interaction. However, virulent SCN populations overcome this incompatibility using unknown mechanisms. A soybean BAG6 (Bcl‐2 associated anthanogene 6) gene previously reported by us to be highly up‐regulated in degenerating feeding sites induced by SCN in a resistant soybean line was attenuated in response to a virulent SCN population. We show that GmBAG6‐1 induces cell death in yeast like its Arabidopsis homolog AtBAG6 and also in soybean. This led us to hypothesize that virulent SCN may target GmBAG6‐1 as part of their strategy to overcome soybean defence responses during infection. Thus, we used a yeast viability assay to screen SCN effector candidates for their ability to specifically suppress GmBAG6‐1‐induced cell death. We identified several effectors that strongly suppressed cell death mediated by GmBAG6‐1. Two effectors identified as suppressors showed direct interaction with GmBAG6‐1 in yeast, suggesting that one mechanism of cell death suppression may occur through an interaction with this host protein.

中文翻译：

大豆胞囊线虫效应子靶向抑制大豆 BAG6 诱导的酵母细胞死亡。

虽然已从细菌、真菌和卵菌病原体中鉴定出许多抑制植物免疫的效应子，但对线虫效应子的了解相对较少。大豆胞囊线虫 (SCN) 中已报道了数十种效应子。先前的研究表明，在不相容的相互作用过程中，大豆线虫进食位点会触发类似程序性细胞死亡的过敏反应。然而，有毒的 SCN 群体使用未知的机制克服了这种不相容性。我们之前报道的大豆BAG6 （Bcl-2 相关 anthanogene 6）基因在抗性大豆品系中由 SCN 诱导的退化摄食位点中高度上调，但在对有毒 SCN 群体的反应中减弱。我们发现 GmBAG6-1 可以诱导酵母（如其拟南芥同源物 AtBAG6）以及大豆中的细胞死亡。这使我们推测，有毒的 SCN 可能以 GmBAG6-1 为目标，作为其克服感染期间大豆防御反应的策略的一部分。因此，我们使用酵母活力测定来筛选 SCN 效应子候选物，以确定其特异性抑制 GmBAG6-1 诱导的细胞死亡的能力。我们鉴定了几种强烈抑制 GmBAG6-1 介导的细胞死亡的效应子。被确定为抑制子的两个效应子显示出与酵母中的 GmBAG6-1 直接相互作用，表明细胞死亡抑制的一种机制可能是通过与该宿主蛋白的相互作用发生的。

更新日期：2020-07-19

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