Neutrophil‐regulated inflammation plays crucial roles in tissue damage and repair. Dysregulation of the neutrophil response system can contribute to diseases such as cancer. Clearance of excessive neutrophils at the site of inflammation by reverse migration provides a promising strategy to mitigate the negative effects. Chlorogenic acid treatment of injured zebrafish embryos showed low‐developmental toxicity. Using a transgenic zebrafish Tg (mpx: egfp) model, chlorogenic acid‐enriched kudingcha extract promoted neutrophil reverse migration via phosphorylation of ERK and AKT. Using i‐TRAQ analysis, differentially expressed proteins involved in focal adhesion were identified, such as: Cdc42, SRC, MLC, ITGA, and Calpain. In support of this, ERK and AKT proteins are involved in the focal adhesion pathway. Real time qPCR determined that CGA downregulates genes associated with cancer metastasis, such as: HSPA5, YWHAZ, RP17, and ITGAV. Together, these results suggest that CGA‐enriched Kudingcha extract may have potential as an anticancer or anti‐inflammatory therapeutic agent.

中文翻译：

---

苦丁茶CJ Tseng茶中富含绿原酸的提取物通过促进通过粘着途径的逆向迁移来抑制斑马鱼中性粒细胞的募集。

中性粒细胞调节的炎症在组织损伤和修复中起关键作用。中性粒细胞反应系统的失调可导致诸如癌症的疾病。通过反向迁移清除炎症部位过量的中性粒细胞提供了减轻不良影响的有前途的策略。绿原酸处理受损斑马鱼胚胎的发育毒性低。使用转基因斑马鱼Tg（mpx：egfp）在模型中，富含绿原酸的苦丁茶提取物通过ERK和AKT的磷酸化促进嗜中性白细胞反向迁移。使用i-TRAQ分析，鉴定了参与粘着斑的差异表达蛋白，例如：Cdc42，SRC，MLC，ITGA和钙蛋白酶。为此，ERK和AKT蛋白参与了粘着斑途径。实时定量PCR确定CGA下调了与癌症转移相关的基因，例如：HSPA5，YWHAZ，RP17和ITGAV。总之，这些结果表明，富含CGA的苦丁茶提取物可能具有抗癌或消炎治疗的潜力。