Cereals and pseudocereals are a rich source of nutrients and trace elements, but their dietary bioavailability is low due to the presence of phytate (IP6), an antinutritional compound with the ability to chelate cations and proteins. Phytase is an enzyme that catalyzes the hydrolysis of IP6 and it is used as an additive improving the nutritional quality of grain‐based foods. The aim of this study was to select lactic acid bacteria (LAB) isolated from pseudocereals with phytase activity, characterize their production and activity, and purify the enzyme. LAB strains isolated from grains and spontaneous sourdough of quinoa and amaranth were grown in the Man Rogosa and Sharpe medium where the inorganic phosphate (Pi) was replaced by 1% of IP6. Phytase activity was determined by measuring the Pi released from IP6. Phytase of Lactobacillus (L.) plantarum CRL1964 (PhyLP) showed the highest specific activity from 73 LAB evaluated. IP6 induces PhyLP production, which is at its maximum at the end of the exponential phase. PhyLP was thermostable and maintained its activity under acidic conditions. The enzymatic activity is stimulated by ethylenediaminetetraacetic acid, Co2+, and ascorbic acid. PhyLP was partially purified and showed a molecular mass of 55 kDa. L. plantarum CRL1964 and/or PhyLP have the potential to be included in the processing of cereal/pseudocereals based products for animal feed and/or the food industry improving its nutritional value.

中文翻译：

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从假谷物中分离的植物乳杆菌 CRL1964 植酸酶的部分表征和纯化

谷物和假谷物是营养物质和微量元素的丰富来源，但由于存在植酸盐 (IP6)，这是一种能够螯合阳离子和蛋白质的抗营养化合物，因此它们的膳食生物利用度较低。植酸酶是一种催化 IP6 水解的酶，它被用作改善谷物食品营养质量的添加剂。本研究的目的是选择从具有植酸酶活性的假谷物中分离的乳酸菌 (LAB)，表征它们的生产和活性，并纯化酶。从谷物和藜麦和苋菜的自发酸面团中分离出的 LAB 菌株在 Man Rogosa 和 Sharpe 培养基中生长，其中无机磷酸盐 (Pi) 被 1% 的 IP6 替代。通过测量从 IP6 释放的 Pi 来确定植酸酶活性。乳酸菌植酸酶 (L. ) 植物源 CRL1964 (PhyLP) 显示出来自评估的 73 个 LAB 的最高比活性。IP6 诱导 PhyLP 产生，其在指数期结束时达到最大值。PhyLP 是热稳定的并在酸性条件下保持其活性。酶活性受到乙二胺四乙酸、Co2+ 和抗坏血酸的刺激。PhyLP 被部分纯化并显示分子量为 55 kDa。植物乳杆菌 CRL1964 和/或 PhyLP 有可能被包括在用于动物饲料和/或食品工业的谷物/伪谷物产品的加工中，以提高其营养价值。PhyLP 是热稳定的并在酸性条件下保持其活性。酶活性受到乙二胺四乙酸、Co2+ 和抗坏血酸的刺激。PhyLP 被部分纯化并显示分子量为 55 kDa。植物乳杆菌 CRL1964 和/或 PhyLP 有可能被包括在用于动物饲料和/或食品工业的谷物/伪谷物产品的加工中，以提高其营养价值。PhyLP 是热稳定的并在酸性条件下保持其活性。酶活性受到乙二胺四乙酸、Co2+ 和抗坏血酸的刺激。PhyLP 被部分纯化并显示分子量为 55 kDa。植物乳杆菌 CRL1964 和/或 PhyLP 有可能被包括在用于动物饲料和/或食品工业的谷物/伪谷物产品的加工中，以提高其营养价值。