Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin,Advanced Science

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Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin
Advanced Science ( IF 14.3 ) Pub Date : 2020-07-19 , DOI: 10.1002/advs.202001365
Byung-Hyun Cha ₁ , Jin-Su Kim _{2,

3} , Alvin Bello ₄ , Geun-Hui Lee ₃ , Do-Hyun Kim ₅ , Byoung Ju Kim ₅ , Yoshie Arai ₅ , Bogyu Choi ₃ , Hansoo Park ₄ , Soo-Hong Lee ₅

Affiliation

Human pluripotent stem cells (hPSCs) are a potent source of clinically relevant mesenchymal stem cells (MSCs) that confer functional and structural benefits in cell therapy and tissue regeneration. Obtaining sufficient numbers of MSCs in a short period of time and enhancing the differentiation potential of MSCs can be offered the potential to improve the regenerative activity of MSCs therapy. In addition, the underlying processes in the isolation and derivation of MSCs from hPSCs are still poorly understood and controlled. To overcome these clinical needs, an efficient and simplified technique on the isolation of MSCs from spontaneously differentiated human embryonic stem cells (hESCs) via integrin α5β1 (fibronectin (FN) receptor)‐to‐FN interactions (hESC‐FN‐MSCs) is successfully developed. It is demonstrated that hESC‐FN‐MSCs exhibit a typical MSC surface phenotype, cellular morphology, with the whole transcriptome similar to conventional adult MSCs; but show higher proliferative capacity, more efficient trilineage differentiation, enhanced cytokine secretion, and attenuated cellular senescence. In addition, the therapeutic potential and regenerative capacity of the isolated hESC‐FN‐MSCs are confirmed by in vitro and in vivo multilineage differentiation. This novel method will be useful in the generation of abundant amounts of clinically relevant MSCs for stem cell therapeutics and regenerative medicine.

中文翻译：

利用整合素α5β1和纤连蛋白之间的相互作用从人胚胎干细胞中有效分离和富集间充质干细胞

人多能干细胞 (hPSC) 是临床相关间充质干细胞 (MSC) 的有效来源，可为细胞治疗和组织再生带来功能和结构益处。在短时间内获得足够数量的MSC并增强MSC的分化潜力可以为提高MSC治疗的再生活性提供潜力。此外，从 hPSC 中分离和衍生 MSC 的基本过程仍然知之甚少和控制。为了克服这些临床需求，我们需要一种高效、简化的技术，通过整合素α 5 β 1（纤连蛋白 (FN) 受体）与 FN 相互作用，从自发分化的人胚胎干细胞 (hESC) 中分离 MSC（hESC-FN-MSC））研制成功。证明hESC-FN-MSCs表现出典型的MSC表面表型、细胞形态，整个转录组与传统成体MSCs相似；但表现出更高的增殖能力、更有效的三系分化、增强的细胞因子分泌和减弱的细胞衰老。此外，分离的 hESC-FN-MSC 的治疗潜力和再生能力通过体外和体内多谱系分化得到证实。这种新方法将有助于产生大量临床相关的 MSC，用于干细胞治疗和再生医学。

更新日期：2020-09-10

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