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Time Domain Nuclear Magnetic Resonance (TD-NMR) to evaluate the effect of potato cell membrane electroporation
Innovative Food Science & Emerging Technologies ( IF 6.3 ) Pub Date : 2020-07-20 , DOI: 10.1016/j.ifset.2020.102456
Ileana Menegazzo , Stefano Mammi , Paolo Sgarbossa , Alessandra Bartolozzi , Mirto Mozzon , Roberta Bertani , Michele Forzan , Raji Sundararajan , Elisabetta Sieni

Time Domain-Nuclear Magnetic Resonance (TD-NMR) was used here for the first time to analyze potato specimens cut from tubers before and after electroporation. The proton transverse relaxation time, T2, was used to identify the modifications that occur at the cell level involving water molecules mobility in potato tubers after the electroporation treatment. Since electroporation modifies the tissue conductivity, the samples were also analyzed in terms of conductivity, water content, and microscopic morphology of the tissue. Comparison analyses were performed on dried potato (zero water content) and water-potato starch mixtures with different water contents. All the data confirm that the effect of the electroporation process can be identified with a variation of the position of the peaks in T2 distribution, associated to sub-cellular modifications.



中文翻译:

时域核磁共振(TD-NMR)评估马铃薯细胞膜电穿孔的作用

在此首次使用时域核磁共振(TD-NMR)分析电穿孔前后从块茎切下的马铃薯标本。质子横向弛豫时间T 2用于鉴定在电穿孔处理后在马铃薯块茎中涉及水分子迁移的细胞水平发生的修饰。由于电穿孔会改变组织的电导率,因此还需要根据电导率,水含量和组织的微观形态对样品进行分析。对干马铃薯(零水含量)和不同水含量的马铃薯淀粉混合物进行了比较分析。所有数据均证实,可以通过改变T 2中峰的位置来识别电穿孔过程的影响 分布,与亚细胞修饰有关。

更新日期:2020-07-23
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