Due to overuse and terrestrial input, there are large quantities of phoxim and prometryne residues in some aquatic environments. In the present study, the effects of these compounds on Penaeus vannamei hepatopancreas were analysed at the transcriptome level to investigate toxicity in this nontarget aquaculture organism. Twelve normalised cDNA libraries were constructed using RNA from phoxim and prometryne treatment groups, and an untreated control group. A total of 667,750,902 clean reads were obtained. Analysis of differentially expressed genes (DEGs) identified 449 in control vs phoxim groups, 185 in control vs prometryne groups, and 183 in prometryne vs phoxim groups. In the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, arachidonic acid metabolism, pancreatic secretion, linoleic acid metabolism, and beta-alanine metabolism pathways were significantly enriched in control vs phoxim groups. In control vs prometryne groups, lysosome, pentose and glucuronate interconversion, antigen processing and presentation, and glycosaminoglycan degradation pathways were significantly enriched. In prometryne vs phoxim groups, protein digestion and absorption, extracellular matrix (ECM)-receptor interaction, PI3K-Akt signalling, cell adhesion molecule (CAM), AGE-RAGE signalling related to diabetic complications, focal adhesion, and renin secretion pathways were significantly enriched. In further detailed analysis, glutathione S-transferase (GST), glutathione peroxidase and basic phospholipase A2 were downregulated in the phoxim treatment group, indicating that phoxim damaged hepatopancreas. Upregulation of phospholipase A2 (secretory phospholipase A2-like) indicates possible inflammatory pathological injury to hepatopancreas caused by phoxim. Meanwhile, downregulation of CD63 indicates that prometryne affect the immune system.

由于过度使用和地面输入，在某些水生环境中存在大量的辛硫磷和异丙基苯残留。在本研究中，这些化合物对南美白对虾的作用在转录组水平上分析了肝胰腺，以研究这种非目标水产养殖生物的毒性。使用来自辛硫磷和炔丙基处理组以及未处理的对照组的RNA构建了十二个标准化的cDNA文库。总共获得667,750,902个干净读取。差异表达基因（DEGs）的分析在对照组与辛硫磷组中确定了449个，在对照组与丙草胺组中为185个，在丙草胺与辛硫磷组中为183个。在《京都市基因与基因组百科全书》（KEGG）途径分析中，对照组和辛硫磷组中花生四烯酸代谢，胰腺分泌，亚油酸代谢和β-丙氨酸代谢途径明显丰富。在对照组和pro丸组中，溶酶体，戊糖和葡糖醛酸的相互转化，抗原加工和呈递，糖胺聚糖降解途径明显丰富。在保萘灵与辛硫磷组中，蛋白质的消化吸收，细胞外基质（ECM）-受体相互作用，PI3K-Akt信号传导，细胞粘附分子（CAM），与糖尿病并发症，局灶性粘附和肾素分泌途径有关的AGE-RAGE信号显着丰富。在进一步的详细分析中，在辛硫磷治疗组中谷胱甘肽S-转移酶（GST），谷胱甘肽过氧化物酶和碱性磷脂酶A2被下调，表明辛硫磷损害了肝胰腺。磷脂酶A2（分泌型磷脂酶A2样）的上调表明辛硫磷可能对肝胰腺造成炎性病理损伤。同时，CD63的下调表明脯氨醇会影响免疫系统。