Graphene oxide (GO) is a derivative of graphene nanosheet which is the most promising material of the decade in biomedical research. In particular, it has been known as an antimicrobial nanomaterial with good biocompatibility. In this study, we have synthesized and characterize GO and checked its antimicrobial property against different Gram-negative and Gram-positive multidrug drug resistant (MDR) hospital superbugs grown in solid agar-based nutrient plates with and without human serum through the utilization of agar well diffusion method, live/dead fluorescent staining and genotoxicity analysis. No significant changes in antibacterial activity were found in these two different conditions. We also compare the bactericidal capability of GO with some commonly administered antibiotics and in all cases the degree of inhibition is found to be higher. The data presented here are novel and show that GO is an effective bactericidal agent against different superbugs and can be used as a future antibacterial agent.

氧化石墨烯（GO）是石墨烯纳米片的衍生物，是近十年来生物医学研究中最有前途的材料。特别是，它被称为具有良好生物相容性的抗菌纳米材料。在这项研究中，我们合成并表征了 GO，并检查了其对不同革兰氏阴性和革兰氏阳性多重耐药 (MDR) 医院超级细菌的抗菌特性，这些超级细菌生长在含有或不含人血清的固体琼脂营养板中，通过使用琼脂孔扩散法、活/死荧光染色和遗传毒性分析。在这两种不同的条件下，没有发现抗菌活性发生显着变化。我们还将 GO 的杀菌能力与一些常用抗生素进行了比较，发现在所有情况下抑制程度都较高。这里提供的数据是新颖的，表明GO是针对不同超级细菌的有效杀菌剂，并且可以用作未来的抗菌剂。