Interleukin 10 (IL-10) is an anti-inflammatory cytokine with lower circulating levels in patients with type 2 diabetes mellitus (T2DM). Cytokines such as IL-10 downregulate the production of pro-inflammatory cytokines, which impair proper function of insulin. So any mutation in the IL-10 gene results in increased production of proinflammatory cytokines, which in turn affect insulin action and cause T2DM. In this study, a polymorphism (rs1800896) in the gene (IL-10) and its association with T2DM was determined with the amplification-refractory mutation system with PCR (ARMS-PCR). Study subjects were divided in two groups, control and T2DM. DNA was extracted from blood, and ARMS-PCR was performed by using specific primers for the promoter region. An amplified product was obtained at 258 base pairs (bp). In the control group, heterozygous bands with both alleles (A/G) were present, whereas AA and GG homozygosity was observed in the T2DM group. This polymorphism (rs1800896) in the IL-10 gene is associated with T2DM. Physical measurements were also obtained, and significant differences between the groups were determined with an independent t-test. Significance was based on a p-value level of 0.05 or less, and highly significant was based on a p-value of 0.01 or less at 95% confidence interval.

中文翻译：

---

白细胞介素10（IL-10）基因与2型糖尿病的关联，其启动子区域G / A 1082的单核苷酸多态性。

白细胞介素10（IL-10）是一种抗炎细胞因子，在2型糖尿病（T2DM）患者中具有较低的循环水平。细胞因子（例如IL-10）下调促炎细胞因子的产生，从而削弱胰岛素的正常功能。因此，IL-10基因中的任何突变都会导致促炎细胞因子的产生增加，进而影响胰岛素作用并引起T2DM。在这项研究中，基因的IL-10多态性（rs1800896）及其与T2DM的关联通过PCR扩增-难治性突变系统（ARMS-PCR）确定。研究对象分为两组，即对照组和T2DM。从血液中提取DNA，并使用针对启动子区域的特异性引物进行ARMS-PCR。获得了258个碱基对（bp）的扩增产物。在对照组中 存在两个等位基因（A / G）的杂合带，而在T2DM组中观察到AA和GG纯合。IL-10基因中的这种多态性（rs1800896）与T2DM相关。还获得了身体测量结果，并通过独立的方法确定了组之间的显着差异t检验 显着性基于0.05或更小的p值水平，高度显着性基于95％置信区间的p值0.01或更小。