Kidney-Yang Deficiency Syndrome (KYDS) is closely bound up with the immune response of immunocompromised patients. The study is to investigate whether retinoic acid-inducible gene-I (RIG-I)-mediated innate immune responseparticipates in the development of KYDS in rats and evaluate the effect of Yougui pill (YGP) on the response in KYDS rats. KYDS rats were induced by intramuscular injection of hydrocortisone at the dose of 10 mg/kg/d for 15 days. YGP at concentrations of 2.43 g/kg/d and 4.86 g/kg/d were administered intragastrically to KYDS rats for 30 days. The results
showed that the body weight, urinary 17-hydroxycorticosteroid (17-OHCS) level, spleen size and spleen index in KYDS rats were significantly decreased compared with healthy control rats, while YGP treatment reversed them towards normal level in a dose-dependent manner. Moreover, KYDS challenge not only strikingly increased the mRNA and protein expression levels of RIG-I, tripartite motif containing 25 (TRIM25), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) but also
markedly enhanced the endogenous RIG-I polyubiquitination levels. Whereas, YGP treatment effectively reversed this tendency in a dose-dependent manner. In conclusion, these findings revealed that RIG-I-mediated innate immune response was closely bound up with the development of KYDS. And YGP exhibited certain anti-inflammatory effects on KYDS rats via inhibiting the RIG-I-mediated innate immune response.


中文翻译：

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肾阳虚证大鼠RIG-I介导的先天免疫反应及其在右归丸给药后的变化分析

肾阳虚综合征（KYDS）与免疫功能低下患者的免疫反应密切相关。该研究旨在研究视黄酸诱导基因-I（RIG-I）介导的先天免疫应答是否参与大鼠KYDS的发展，并评价尤归丸（YGP）对KYDS大鼠反应的影响。通过肌肉注射氢化可的松以10 mg / kg / d的剂量诱导KYDS大鼠15天。将YGP分别以2.43g / kg / d和4.86g / kg / d的浓度经胃内给予KYDS大鼠30天。结果
结果显示，与健康对照组相比，KYDS大鼠的体重，尿液中的17-羟基皮质类固醇（17-OHCS）水平，脾脏大小和脾脏指数显着降低，而YGP治疗则使它们以剂量依赖的方式逆转至正常水平。此外，KYDS攻击不仅显着提高了RIG-I，包含25（TRIM25）的三方基序，肿瘤坏死因子-α（TNF-α）和白介素6（IL-6）的mRNA和蛋白质表达水平，而且还显着提高了
显着提高了内源性RIG-1泛素化水平。然而，YGP治疗以剂量依赖性方式有效逆转了这种趋势。总之，这些发现表明，RIG-I介导的先天免疫反应与KYDS的发展密切相关。YGP通过抑制RIG-I介导的先天免疫反应，对KYDS大鼠具有一定的抗炎作用。