Kanocha is the dried seeds of Phyllanthus maderaspatensis of family Phyllanthaceae. It has been long utilising in Unani system of medicine to cure many ailments including genitourinary infectious diseases. In the current investigation, the aqueous, alcoholic and hydroalcoholic extracts of Kanocha seeds were screened for their antibacterial actions against both gram-positive (Streptococcus mutans, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Corynebacterium xerosis and Bacillus cereus) and gram-negative (Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris) bacterial strains compared with the standard Drug: Ciprofloxacin (SD060) 5 μg/disk for gram-positive bacterial strains and Gentamicin (SD016) 10 μg/disk for gram-negative bacterial strains using Zone of Inhibition (ZOI) with the help of Agar well method and minimum inhibitory concentration (MIC) & minimum bactericidal concentration (MBC) with the help of Nutrient Broth method. The data was analysed using Gpad INSTAT programming, one-way ANOVA and post-test Bonferroni. Alcoholic and hydroalcoholic extract showed significant antibacterial activity than the aqueous extract but not up to the mark as compared to standard group indicating that the alcoholic and hydroalcoholic extract has the capability of extracting more phytochemicals than aqueous extract which are responsible for
their antibacterial activity. It could be concluded that the present drug possesses antibacterial property.


中文翻译：

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Kanocha种子（Phyllanthus maderaspatensis）对某些革兰氏阳性和革兰氏阴性细菌菌株的体外抗菌活性研究

Kanocha是竹兰科的Phyllanthus maderaspatensis的干燥种子。在Unani的医学系统中长期使用它来治疗包括泌尿生殖道传染病在内的许多疾病。在当前的调查中，筛选了Kanocha种子的水，酒精和水醇提取物对革兰氏阳性（变形链球菌，金黄色葡萄球菌，表皮葡萄球菌，化脓性链球菌，干燥杆菌和干燥杆菌）的抑菌作用。与标准药物相比，肺炎克雷伯菌，大肠埃希菌，铜绿假单胞菌和寻常变形杆菌的细菌菌株：环丙沙星（SD060）5克/片用于革兰氏阳性细菌菌株，庆大霉素（SD016）10克/片用于革兰氏阴性细菌菌株，使用琼脂井法和最小抑菌浓度（MIC）借助抑制区（ZOI）借助营养肉汤法实现最低杀菌浓度（MBC）。使用Gpad INSTAT编程，单向方差分析和后测Bonferroni分析数据。醇和水醇提取物显示出比水提取物显着的抗菌活性，但与标准组相比未达到标记，表明醇和水醇提取物比水提取物具有更多的植物化学物质提取能力。借助营养肉汤法测定最低杀菌浓度（MBC）。使用Gpad INSTAT编程，单向方差分析和后测Bonferroni分析数据。醇和水醇提取物显示出比水提取物显着的抗菌活性，但与标准组相比未达到标记，表明醇和水醇提取物比水提取物具有更多的植物化学物质提取能力。借助营养肉汤法测定最低杀菌浓度（MBC）。使用Gpad INSTAT编程，单向方差分析和后测Bonferroni分析数据。醇和水醇提取物显示出比水提取物显着的抗菌活性，但与标准组相比未达到标记，表明醇和水醇提取物比水提取物具有更多的植物化学物质提取能力。
它们的抗菌活性。可以得出结论，本发明的药物具有抗菌性能。