. We report here the first molecular evidence for the occurrence of Aricia artaxerxes (Fabricius, 1793) (Lepidoptera: Lycaenidae) in the Czech Republic. In Central Europe, this species may co-occur with its more common sibling, Aricia agestis (Denis & Schiffermüller, 1775). We sequenced the cytochrome c oxidase subunit 1 of darkly-coloured, putative A. artaxerxes specimens in the Czech Republic. We confirmed A. artaxerxes only from a limestone area in South Bohemia (Vyšenské kopce National Nature Reserve), which is probably the only locality of the species in the Czech Republic. This area is located at ca. 550 m A.S.L., showing that the elevation overlap with A. agestis could be high in Central Europe. Other surveyed individuals were confirmed as A. agestis, with a minimum p-distance of 1.98% between the two species. The South Bohemian area of occurrence is probably highly isolated (approx. 190 km) from localities of the species in neighbouring countries, highlighting the conservation importance of the A. artaxerxes population and of the insular calcareous areas in the Šumava Mountains foothills. We used database sequences of A. artaxerxes to place the Czech population into a wider phylogeographic context. The Czech population is monomorphic, consisting of a single haplotype, which is present from Scandinavia through Germany to Central Asia.

中文翻译：

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捷克共和国Aricia artaxerxes（Fabricius，1793）（鳞翅目，Lycaenidae）的遗传学证实，其保护意义和生物地理背景

。我们在此报告捷克共和国Aricia artaxerxes（Fabricius，1793）（鳞翅目：Lycaenidae）发生的第一份分子证据。在中欧，该物种可能与其更常见的同胞Aricia agestis同时出现（Denis＆Schiffermüller，1775年）。我们在捷克共和国对深色，假定的青蒿的标本中的细胞色素c氧化酶亚基1进行了测序。我们仅在南波希米亚的石灰岩地区（Vyšenskékopce国家自然保护区）证实了青蒿。这可能是捷克共和国该物种的唯一产地。该区域位于大约。550 m ASL，表明在中欧与A. agestis重叠的高程可能很高。其他被调查个体被确认为A. agestis，两个物种之间的最小p距离为1.98％。南波希米亚的发生地区可能与邻近国家的物种高度隔离（约190 km），突显了青蒿A. axerxes种群和Šumava山山麓小岛钙质地区的保护重要性。我们使用了A. artaxerxes的数据库序列来将捷克人群置于更广泛的系统地理环境中。捷克人口是单态的，由一个单倍型组成，从斯堪的纳维亚半岛到德国再到中亚。artaxerxes将捷克人置于更广泛的系统地理环境中。捷克人口是单态的，由一个单倍型组成，从斯堪的纳维亚半岛到德国再到中亚。artaxerxes将捷克人置于更广泛的系统地理环境中。捷克人口是单态的，由一个单倍型组成，从斯堪的纳维亚半岛到德国再到中亚。