This study aims to investigate the production of scaffolds by selective laser sintering (SLS) using poly(vinyl alcohol) (PVA) polymer, for in vitro studies, a relatively new and growing area in which scaffolds could be used in the design of three-dimensional models for in vitro disease model or tissue equivalent for safety and effectiveness tests.,The influence of the SLS process parameters laser power, 26 W and 32 W, and number of laser scans, 1, 2, 4 and 6, on the surface microstructure of the samples and on the degree of crystallinity and chemical stability of PVA material, was investigated using powder with particle size of 20-320 µm. Laser sintered PVA samples were subjected to cell culture tests using osteoblastic cells derived from human osteosarcoma (SaOs-2).,The laser power has no significant influence on the microstructure of the laser-sintered samples, however the number of scans has a considerable influence on the sintering degree; the SLS process causes a decrease in the degree of crystallinity and changes the chemical structure of the as-received PVA, especially when using higher laser power and more number of scans. Preliminary in vitro cell culture tests show that the laser-sintered PVA material is biocompatible with SaOs-2 cells.,SLS offers good potential for the fabrication of scaffolds and thus, may be applied as an alternative to conventional scaffold fabrication processes to overcome their limitations.

中文翻译：

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选择性激光烧结工艺参数对制备支架用聚乙烯醇的微观结构和理化性能的影响

本研究旨在研究使用聚乙烯醇 (PVA) 聚合物通过选择性激光烧结 (SLS) 生产支架的体外研究，这是一个相对较新且不断增长的领域，其中支架可用于设计三用于安全性和有效性测试的体外疾病模型或组织等效物的维度模型。SLS 工艺参数激光功率 26 W 和 32 W 以及激光扫描次数 1、2、4 和 6 对表面的影响使用粒径为 20-320 µm 的粉末研究样品的微观结构以及 PVA 材料的结晶度和化学稳定性。使用源自人骨肉瘤 (SaOs-2) 的成骨细胞对激光烧结的 PVA 样品进行细胞培养测试。激光功率对激光烧结样品的微观结构没有显着影响，而扫描次数对烧结程度有相当大的影响；SLS 工艺会导致结晶度降低并改变原样 PVA 的化学结构，尤其是在使用更高的激光功率和更多的扫描次数时。初步的体外细胞培养测试表明，激光烧结的 PVA 材料与 SaOs-2 细胞具有生物相容性。SLS 为支架的制造提供了良好的潜力，因此，可用作传统支架制造工艺的替代品，以克服其局限性. SLS 工艺会导致结晶度降低并改变原样 PVA 的化学结构，尤其是在使用更高的激光功率和更多的扫描次数时。初步的体外细胞培养测试表明，激光烧结的 PVA 材料与 SaOs-2 细胞具有生物相容性。SLS 为支架的制造提供了良好的潜力，因此，可用作传统支架制造工艺的替代品，以克服其局限性. SLS 工艺会导致结晶度降低并改变原样 PVA 的化学结构，尤其是在使用更高的激光功率和更多的扫描次数时。初步的体外细胞培养试验表明，激光烧结的 PVA 材料与 SaOs-2 细胞具有生物相容性。SLS 为支架的制造提供了良好的潜力，因此，可用作传统支架制造工艺的替代品，以克服其局限性.