A HPLC method was developed and validated to analyze meropenem and vaborbactam simultaneously in murine plasma and saline matrixes. A 60-μL volume of extracted sample was injected onto a 5-μm BDS Phenyl-Hypersil C₁₈ reversed-phase column and analyzed with a UV detector set at 298 nm for the first 4.9 min and switched to 240 nm. The mobile phase contained a mixture of methanol and 25-mM sodium phosphate buffer set at a flow rate of 1.0 mL/min for the 16 min run time. Cefuroxime was used as the internal standard. The standard curves were linear over a range of 0.25–50 μg/mL. The precision and accuracy for 0.25 μg/mL (LLQ) in plasma for both compounds were <4.8% and >98.9%, respectively. Interday and intraday precision and accuracy for all QC plasma samples for both compounds were <6.2% and >95.7%, respectively. This methodology details a reproducible assay for both compounds using a single extraction with good accuracy and precision.

中文翻译：

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用于测定生物和水性基质中美洛培南/ Vaborbactam的HPLC方法的开发。

建立了HPLC方法，并验证了该方法可同时分析鼠血浆和生理盐水中的美罗培南和维巴巴坦。将60μL体积的提取样品注入5μmBDS Phenyl-Hypersil C ₁₈反相柱，在开始的4.9分钟内用设置在298 nm的UV检测器进行分析，并切换至240 nm。在16分钟的运行时间内，流动相包含甲醇和25-mM磷酸钠缓冲液的混合物，流速设置为1.0 mL / min。头孢呋辛用作内标。标准曲线在0.25–50μg/ mL范围内呈线性。两种化合物的血浆中0.25μg/ mL（LLQ）的精密度和准确度分别为<4.8％和> 98.9％。两种化合物的所有QC血浆样品的日间和日内精度和准确度分别为<6.2％和> 95.7％。该方法论详细描述了使用一次提取以良好的准确性和精密度对两种化合物进行可重现的测定方法。