Abstract

Background

Docosahexaenoic acid (DHA) plays an important role in brain and retinal development in dogs. However, supranutritional dietary supplementation can result in health issues, including gastrointestinal bleeding, making the accurate analysis of DHA in dog food important for nutritional and welfare regulatory compliance.

Objective

The aim of this study was to conduct a validation and verification of the AOAC 996.06 method, and hence establish its fitness for purpose, for the analysis of DHA in dried dog food supplemented with a heterotrophically grown unextracted DHA-rich Aurantiochytrium limacinum biomass.

Methods

The AOAC 996.06 method, which involves the use of gas chromatography coupled to flame ionization detection (GC-FID), was used to conduct a validation of the analysis of DHA in dried dog food and the results were verified in a second laboratory.

Results

The method was found to be linear over the ranges analyzed and results were found to be within the acceptance criteria for precision and accuracy, verifying the applicability for this matrix. The selectivity and sensitivity of the method were also determined.

Conclusions

The AOAC 996.06 method is fit for purpose for the analysis of DHA in dry dog food kibble.

Highlights

The method can be applied to various dog food samples, supplemented with an unextracted Aurantiochytrium limacinum biomass, using alternative manufacturing methods, i.e. pelleted and extruded with no significant matrix effects being observed.

中文翻译：

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富含 Aurantiochytrium limacinum 生物质的干狗粮中二十二碳六烯酸 (DHA) 的分析：矩阵扩展验证和 AOAC 方法 996.06 的验证

摘要

背景

二十二碳六烯酸 (DHA) 在狗的大脑和视网膜发育中发挥着重要作用。然而，超营养膳食补充剂可能会导致健康问题，包括胃肠道出血，因此准确分析狗粮中的 DHA 对于营养和福利监管合规性非常重要。

客观的

本研究的目的是对 AOAC 996.06方法进行验证和验证，从而确定其适用性，用于分析补充有异养生长的未提取的富含 DHA Aurantiochytrium limacinum生物质的干狗粮中的 DHA。

方法

AOAC 996.06方法涉及使用气相色谱与火焰离子化检测 (GC-FID)，对干狗粮中的 DHA 分析进行验证，并在第二个实验室验证结果。

结果

该方法在分析范围内呈线性，结果在精度和准确度的可接受标准内，验证了该矩阵的适用性。还确定了该方法的选择性和灵敏度。

结论

AOAC 996.06方法适合分析干狗粮粗粒中的 DHA。

强调

该方法可应用于各种狗粮样品，辅以未提取的Aurantiochytrium limacinum生物质，使用替代制造方法，即造粒和挤出，没有观察到显着的基质效应。