To unlock the potential of Chlorella stigmatophora (Trebouxiophyceae, Chlorophyta) and Hemiselmis andersenii (Cryptophyceae, Cryptophyta) as natural reactors for biotechnological exploitation, their lipophilic extracts were characterized using Fourier Transform Infrared spectroscopy with Attenuated Total Reflectance (FTIR‐ATR) and Gas Chromatography‐Mass Spectrometry (GC‐MS) before and after alkaline hydrolysis. The GC‐MS analysis enabled the identification of 62 metabolites—namely fatty acids (27), aliphatic alcohols (17), monoglycerides (7), sterols (4), and other compounds (7). After alkaline hydrolysis, monounsaturated fatty acids increased by as much as 87%, suggesting that the esterified compounds were mainly neutral lipids. Hemiselmis andersenii yielded the highest Σω3/Σω6 ratio (7.26), indicating that it is a good source of ω3 fatty acids, in comparison to C. stigmatophora (Σω3/Σω6 = 1.24). Both microalgae presented significant amounts of aliphatic alcohols (6.81–10.95 mg · g dw⁻¹), which are recognized by their cholesterol‐lowering properties. The multivariate analysis allowed visualization of the chemical divergence among H. andersenii lipophilic extracts before and after alkaline hydrolysis, as well as species‐specific differences. Chlorella stigmatophora showed to be a valuable source of essential fatty acids for nutraceuticals, whereas H. andersenii, due to its high chemical diversity, seems to be suitable for different fields of application.

中文翻译：

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Hemiselmis andersenii和Chlorella stigmatophora作为高价值化合物的新来源：一种脂质组学方法。

为了释放小球藻（Trebouxiophyceae，Chlorophyta）和Hemiselmis andersenii（Cryptophyceae，Cryptophyta）作为生物技术开发天然反应器的潜力，使用具有衰减全反射和FTIR的傅立叶变换红外光谱（FTIR-ATR）对亲脂性提取物进行了表征。碱水解前后的质谱（GC-MS）GC-MS分析能够鉴定出62种代谢物，即脂肪酸（27），脂肪族醇（17），甘油单酸酯（7），固醇（4）和其他化合物（7）。碱水解后，单不饱和脂肪酸增加多达87％，表明酯化的化合物主要是中性脂质。安德森氏菌产生最高Σω3/Σω6比（7.26），这表明它是ω3脂肪酸的良好来源，相较于C. stigmatophora（Σω3/Σω6= 1.24）。两种微藻都表现出大量的脂肪醇（6.81–10.95 mg·g dw ^-1），它们具有降低胆固醇的特性。多元分析允许可视化碱水解之前和之后安徒生嗜油菌提取物之间的化学差异以及物种特异性差异。小球藻 stigmatophora显示是必需脂肪酸的保健食品的重要来源，而H. andersenii由于其高度的化学多样性，似乎适合于不同的应用领域。