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Modeling the combined effect of high hydrostatic pressure and mild heat on the sub‐lethal injury of Listeria monocytogenes by Box–Behnken design
Journal of Food Process Engineering ( IF 2.7 ) Pub Date : 2020-07-19 , DOI: 10.1111/jfpe.13480
Hua Zhu 1, 2 , Yanyan Xu 3 , Guohong Qi 2 , Suilou Wang 2 , Haixiang Wang 2
Affiliation  

In this study, a predictive model based on the Box–Behnken design of response surface methodology was applied to investigate the effects of high hydrostatic pressure (300, 400 and 500 MPa), temperature (20, 30 and 40°C) and treatment time (5, 10 and 15 min) on the sub‐lethal injury of Listeria monocytogenes (L. monocytogenes). The percentage of sub‐lethal injury was calculated by the difference in L. monocytogenes counts observed on nonselective and selective media. The established predictive model presented a goodness of fit to the experimental data (p < .001) with high correlation coefficient value (R2 = .9999) and adjusted correlation coefficient value (Adj‐R2 = .9995), along with an insignificant lack‐of‐fit term (p = .4299). The synergistic effects of pressure, temperature and treatment time on the sub‐lethal injury of L. monocytogenes were found within the tested experimental ranges. Results indicated that the established model has the potential for predicting the effects of high hydrostatic pressure processing on the sub‐lethal injury of L. monocytogenes.

中文翻译:

通过Box–Behnken设计模拟高静水压力和温和热量对单核细胞增生李斯特菌亚致死性损伤的联合作用

在这项研究中,基于响应面方法Box-Behnken设计的预测模型用于研究高静水压力(300、400和500 MPa),温度(20、30和40°C)和处理时间的影响(5、10和15分钟)对单核细胞增生李斯特菌L. monocytogenes)的亚致死性伤害。通过在非选择性和选择性培养基上观察到的单核细胞增生李斯特氏菌计数差异来计算亚致死性损伤的百分比。建立的预测模型 具有较高的相关系数值(R 2 = .9999)和调整后的相关系数值(Adj‐ R 2)与实验数据(p <.001)拟合良好= .9995),以及无关紧要的失恋期(p = .4299)。在测试的实验范围内,发现压力,温度和处理时间对单核细胞增生李斯特菌亚致死性损伤的协同作用。结果表明,建立的模型具有预测高静水压力处理对单核细胞增生李斯特菌亚致死性损伤的潜力。
更新日期:2020-07-19
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