A new unique enolic glucoside of phenylpyruvic acid, carthamusuchuric acid (1), and five known phenolic compounds (2–6) were isolated from the florets of Carthamus tinctorius. The structure of the new compound was elucidated by extensive spectroscopic analyses using one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy, high-resolution electrospray ionization mass spectrometry, and enzymatic hydrolysis. The anti-adipogenic activity of compounds 1–6 was evaluated by staining lipid droplets in 3T3-L1 preadipocytes with Oil Red O and assessing mRNA expression of adipocyte markers. The results showed that compounds 2 and 4 inhibited the differentiation of 3T3-L1 preadipocytes to adipocytes.

苯丙酮酸的一种新的独特的烯醇葡糖苷，carthamusuchuric酸（1）和5种已知的酚类化合物（2 - 6）从的小花分离红花。使用一维和二维核磁共振波谱，高分辨率电喷雾电离质谱和酶水解，通过广泛的光谱分析来阐明新化合物的结构。化合物的抗脂肪生成活性1 - 6通过在3T3-L1前脂肪细胞用油红O染色脂肪滴和评估脂肪细胞标记物的mRNA的表达进行评价。结果表明，化合物2和4 抑制3T3-L1前脂肪细胞向脂肪细胞的分化。