The molecular mechanism of stress-induced immunosuppression (SIS) in certain poultry immune organs is not completely clear. In this study, we constructed a stress immunosuppression model by selecting 180 healthy 7-day-old Gushi chickens and dividing them randomly into two groups: a D_T group and a B_T group. The D_T group was given dexamethasone, and the B_T group was given normal saline, according to the treatment method established and reported in our previous study. Thymus samples were subsequently taken from both groups. RNA-seq was used to sequence the transcriptomes of the thymus samples from both groups, and 1278 significant differentially expressed genes (DEGs) were obtained, of which 845 genes were up-regulated and 433 genes were down-regulated (padj<0.05, |FC| ≥ 2, FPKM>1). We identified immune-related gene ontology (GO) terms including immune system processes, immune system process regulation, and T cell activation. The results of KEGG (http: //www.kegg.jp) analysis showed that the DEGs are involved in a variety of immune-related pathways, such as cytokine-cytokine receptor interactions, Jak-STAT signaling pathways, and cell adhesion molecules (CAMs). The cytokine-cytokine receptor interaction pathway involves the DEGs CCR6, CCR5, CD40LG and FAS. The DEGs in the Jak-STAT signaling pathway were SPRY2, BCL2L1. These DEGS play an important role in cell apoptosis. CD40L, CD8, among other genes, are involved in the CAMs pathway. The results of this study add to existing data on the genomic study of stress affecting immune function, and provide a basis for further studies of the molecular mechanisms of stress-influenced immune function.

某些家禽免疫器官中应激诱导的免疫抑制（SIS）的分子机制尚不完全清楚。在这项研究中，我们通过选择180只7日龄健康的固始鸡并将它们随机分为两组：D_T组和B_T组，构建了应激免疫抑制模型。根据我们先前研究中建立和报道的治疗方法，D_T组给予地塞米松，B_T组给予生理盐水。随后从两组中采集胸腺样品。用RNA-seq对两组胸腺样品的转录组进行测序，获得了1278个显着差异表达基因（DEGs），其中845个基因上调且433个基因下调（padj <0.05，| p <0.05）。 FC |≥2，FPKM> 1）。我们确定了免疫相关基因本体论（GO）术语，包括免疫系统过程，免疫系统过程调节和T细胞活化。KEGG（http://www.kegg.jp）分析的结果表明，DEG参与了多种与免疫相关的途径，例如细胞因子与细胞因子受体的相互作用，Jak-STAT信号传导途径以及细胞粘附分子（ CAMs）。细胞因子-细胞因子受体相互作用途径涉及DEGs CCR6，CCR5，CD40LG和FAS。Jak-STAT信号通路中的DEG是SPRY2，BCL2L1。这些DEGS在细胞凋亡中起重要作用。CD40L，CD8等基因均参与CAM途径。这项研究的结果增加了有关影响免疫功能的应激基因组研究的现有数据，