Abstract Objective The aim of this study was to evaluate the deproteinizing/pretreatment effect of enzyme solutions on cavity adaptation and bond strengths to enamel and dentin. Materials and methods Class V cavities with enamel and dentin margins were prepared using bovine incisors. After one of four pre-treatments (bromelain, papain, HOCl-solution and none), Scotchbond Universal (SU) or Clearfil Universal Bond Quick (UQ) was applied to the cavities, followed by filling resin composite. After water storage for 24 h, the interfacial gap lengths were calculated using SS-OCT. In addition, the micro-shear bond strength (μSBS) test to flat enamel and dentin surfaces treated as mentioned above was performed. Furthermore, the enamel and dentin surfaces after the pretreatments were morphologically investigated using a scanning electronic microscopy (SEM). Results The pretreatment of bromelain and HOCl-solutions significantly increased μSBS of SU to enamel and dentin, and improved enamel-marginal and internal adaptations in the cavity (p 0.05) although they significantly increased μSBS to dentin (p 0.05). SEM observations revealed that treatments of bromelain and HOCl-solutions partially removed the dentin smear layer, while papain treatment slightly thinned it. Conclusion Pretreatment with bromelain could increase μSBS to enamel and dentin, and improve the cavity adaptation due to partial dissolution of the organic phase in the smear layer.

中文翻译：

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酶液涂抹层脱蛋白对一步法自酸蚀胶粘接效果的影响

摘要 目的本研究的目的是评估酶溶液的脱蛋白/预处理对牙釉质和牙本质的腔适应和粘合强度的影响。材料和方法 使用牛门牙制备具有牙釉质和牙本质边缘的 V 级蛀牙。在四种预处理（菠萝蛋白酶、木瓜蛋白酶、HOCl 溶液和无）中的一种后，将 Scotchbond Universal (SU) 或 Clearfil Universal Bond Quick (UQ) 应用于空腔，然后填充树脂复合材料。储水 24 小时后，使用 SS-OCT 计算界面间隙长度。此外，对如上所述处理的平坦牙釉质和牙本质表面进行微剪切粘合强度（μSBS）测试。此外，使用扫描电子显微镜（SEM）对预处理后的牙釉质和牙本质表面进行形态学研究。结果菠萝蛋白酶和HOCl 溶液的预处理显着增加了SU 对牙釉质和牙本质的μSBS，并改善了牙釉质-边缘和腔内的内部适应性（p = 0.05），尽管它们显着增加了对牙本质的μSBS（p = 0.05）。SEM 观察显示菠萝蛋白酶和 HOCl 溶液的处理部分去除了牙本质污迹层，而木瓜蛋白酶处理使其稍微变薄。结论 菠萝蛋白酶预处理可增加对牙釉质和牙本质的μSBS，并由于涂抹层中有机相的部分溶解而改善腔适应。SEM 观察显示菠萝蛋白酶和 HOCl 溶液的处理部分去除了牙本质污迹层，而木瓜蛋白酶处理使其稍微变薄。结论 菠萝蛋白酶预处理可增加对牙釉质和牙本质的μSBS，并由于涂抹层中有机相的部分溶解而改善腔适应。SEM 观察显示菠萝蛋白酶和 HOCl 溶液的处理部分去除了牙本质污迹层，而木瓜蛋白酶处理使其稍微变薄。结论 菠萝蛋白酶预处理可以增加对牙釉质和牙本质的μSBS，并由于涂抹层中有机相的部分溶解而改善腔适应。