Abstract Zein nanoparticles have good prospects as a pharmaceutical carrier but limited in further application by its poor stability and re-dispersibility. To obtain high stability and pH-responsive drug delivery, succinylated-zein (Suc-zein) was synthesized by nucleophilic substitution reaction, the material obtained was used to prepare Suc-zein nanoparticles (SZs). The SZs prepared not only had good dispersibility but showed better stability than zein particles on pH, storage and salinity. After loading indomethacin (Indo) into zeolitic imidazolate framework-8 (ZIF-8), the core-shelled Indo-Suc-zein-ZIF-8 (Indo-SZZs) were fabricated by coating Suc-zein onto the surface of ZIF-8 with better stability and high drug loading. Indo-SZZs were pH-responsive and sustained a drug release profile in a simulated environment. Also SZZs presented as being non-toxic, having good biocompatibility and good cellular up-take performance, and the cytotoxicity of Indo-SZZs was significantly higher than that of Indo. This provides a new insight into the preparation of a zein-based oral drug delivery system.

中文翻译：

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琥珀酰化-玉米醇溶蛋白-ZIF-8 杂化物的简便制备，用于增强稳定性和 pH 响应性药物递送

摘要 玉米醇溶蛋白纳米粒作为药物载体具有良好的前景，但其稳定性和再分散性较差，限制了进一步应用。为了获得高稳定性和pH响应性药物递送，通过亲核取代反应合成琥珀酰化玉米醇溶蛋白（Suc-zein），所得材料用于制备Suc-zein纳米颗粒（SZs）。制备的 SZs 不仅具有良好的分散性，而且在 pH、储存和盐度方面表现出比玉米蛋白颗粒更好的稳定性。将吲哚美辛 (Indo) 加载到沸石咪唑酯骨架-8 (ZIF-8) 中后，通过将 Suc-zein 涂覆到 ZIF-8 表面制备核壳 Indo-Suc-zein-ZIF-8 (Indo-SZZs)具有更好的稳定性和高载药量。Indo-SZZs 对 pH 值有反应，并在模拟环境中保持药物释放曲线。SZZs还表现出无毒、具有良好的生物相容性和良好的细胞吸收性能，并且Indo-SZZs的细胞毒性明显高于Indo。这为制备基于玉米醇溶蛋白的口服给药系统提供了新的见解。