Transmembrane delivery of biomolecules through nanoparticles plays an important role in targeted therapy. Here, we designed a simple nanoparticle for the delivery of model peptide drug into primary osteoclast precursor cells (bone marrow macrophages) by thermosensitive and biodegradable diblock copolymer monomethoxy poly(ethylene glycol)-block-poly(trimethylene carbonate). The model peptide drug was encapsulated into the nanoparticle by dropping the drug carrier dissolved in dimethylsulfoxide solvent into water containing poly(vinyl alcohol) to achieve temperature response nanoparticles. Through size analysis, we found that the nanoparticles possessed a temperature-sensitive property between 30°C and 40°C. Moreover, flow cytometry and spectrofluorimetry analysis indicated that nanoparticle systems underwent significant cellular uptake. In addition, the evaluation of cell biology showed that nanoparticles have excellent biocompatibility. Thus, the results indicated that the temperature-sensitive nanoparticles have potential application value for targeted delivery of oligopeptide in the treatment process of osteoarthritis.

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用于将寡肽递送到破骨细胞前体中的 mPEG-PTMC 热敏纳米颗粒

通过纳米粒子跨膜传递生物分子在靶向治疗中起着重要作用。在这里，我们设计了一种简单的纳米颗粒，用于通过热敏和可生物降解的二嵌段共聚物单甲氧基聚（乙二醇）-嵌段-聚（碳酸三亚甲基酯）将模型肽药物递送到原代破骨细胞前体细胞（骨髓巨噬细胞）中。通过将溶解在二甲亚砜溶剂中的药物载体滴入含有聚乙烯醇的水中，将模型肽药物封装到纳米颗粒中，以实现温度响应纳米颗粒。通过尺寸分析，我们发现纳米颗粒在 30°C 到 40°C 之间具有温度敏感特性。此外，流式细胞术和荧光光谱分析表明纳米颗粒系统经历了显着的细胞摄取。此外，细胞生物学评估表明纳米颗粒具有优异的生物相容性。因此，结果表明温度敏感纳米颗粒在寡肽靶向递送在骨关节炎治疗过程中具有潜在的应用价值。