Objective. To investigate the relationship between pain behavior and potassium voltage-gated channel subfamily A member 2 (KCNA2) expression in dorsal root ganglia (DRGs) of rats with osteoarthritis (OA). Methods. Male Sprague-Dawley rats were randomly divided into three groups: blank control group (group C), normal saline group (group S), and group OA. Paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured one day before injection and one, two, four, and six weeks after injection. At one, two, four, and six weeks after injection, pathological knee joint changes and activated transcription factor-3 (ATF-3) and KCNA2 expressions in DRGs were analyzed. Results. Compared with preinjection, PWMT and PWTL at two, four, and six weeks after injection were significantly decreased in the group OA ( or 0.01). Compared with group C, PWMT and PWTL at two, four, and six weeks after injection were significantly decreased in the group OA ( or 0.01). In the group OA, slight local articular cartilage surface destruction was found at week one. The cartilage surface destruction gradually developed, and the exacerbation of cartilage matrix reduction and bone hyperplasia were increasingly aggravated and eventually evolved into advanced OA in the second to sixth weeks. Compared with group C, ATF-3 expression was significantly increased, and KCNA2 expression was significantly decreased in the group OA at two, four, and six weeks after injection ( or 0.01). Compared to baseline, ATF-3 expression was significantly increased, and KCNA2 expression was significantly decreased in the group OA ( or 0.01). Conclusion. Pain behavior in OA rats was associated with decreased KCNA2 expression in DRGs.

中文翻译：

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骨关节炎大鼠疼痛行为与背根神经节中KCNA2表达变化的关系

目标。探讨疼痛行为与骨关节炎（OA）大鼠背根神经节（DRGs）中钾电压门控通道亚家族A成员2（KCNA2）表达之间的关系。方法。将雄性Sprague-Dawley大鼠随机分为三组：空白对照组（C组），生理盐水组（S组）和OA组。在注射前一天以及注射后一，二，四和六周时测量爪缩机械阈值（PWMT）和爪缩热潜伏期（PWTL）。注射后1、2、4和6周，分析了DRG中的病理性膝关节变化以及活化的转录因子3（ATF-3）和KCNA2表达。结果。与注射前相比，OA组在注射后两周，四周和六周的PWMT和PWTL显着降低（或0.01）。与C组相比，OA组在注射后2、4和6周的PWMT和PWTL显着降低（或0.01）。在OA组中，在第一周发现轻微的局部关节软骨表面破坏。软骨表面破坏逐渐发展，软骨基质减少和骨质增生的恶化加剧，并在第二至第六周发展为晚期OA。与C组相比，OA组在注射后2周，4周和6周时，ATF-3表达显着增加，而KCNA2表达则显着下降（或0.01）。与基线相比，OA组中ATF-3表达显着增加，而KCNA2表达显着降低（或0.01）。结论。OA大鼠的疼痛行为与DRGs中KCNA2表达降低有关。