Tubular Fibrous Scaffolds Functionalized with Tropoelastin as a Small-Diameter Vascular Graft.,Biomacromolecules

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Tubular Fibrous Scaffolds Functionalized with Tropoelastin as a Small-Diameter Vascular Graft.
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-07-17 , DOI: 10.1021/acs.biomac.0c00599
Sofia Oliveira _{1,

2} , Tatiana Felizardo _{1,

2} , Sara Amorim _{1,

2} , Suzanne M Mithieux _{3,

4} , Ricardo A Pires _{1,

2} , Rui L Reis _{1,

2} , Albino Martins _{1,

2} , Anthony S Weiss _{3,

4,

5} , Nuno M Neves _{1,

2}

Affiliation

Cardiovascular disorders are a healthcare problem in today’s society. The clinically available synthetic vascular grafts are thrombogenic and could induce intimal hyperplasia. Rapid endothelialization and matched mechanical properties are two major requirements to be considered when designing functional vascular grafts. Herein, an electrospun tubular fibrous (eTF) scaffold was biofunctionalized with tropoelastin at the luminal surface. The luminal surface functionalization was confirmed by an increase of the zeta potential and by the insertion of NH₂ groups. Tropoelastin was immobilized via its −NH₂ or −COOH groups at the activated or aminolysed eTF scaffolds, respectively, to study the effect of exposed functional groups on human endothelial cells (ECs) behavior. Tensile properties demonstrated that functionalized eTF scaffolds presented strength and stiffness within the range of those of native blood vessels. Tropoelastin immobilized on activated eTF scaffolds promoted higher metabolic activity and proliferation of ECs, whereas when immobilized on aminolysed eTF scaffolds, significantly higher protein synthesis was observed. These biofunctional eTF scaffolds are a promising small-diameter vascular graft that promote rapid endothelialization and have compatible mechanical properties.

中文翻译：

用Tropoelastin作为小直径血管移植物功能化的管状纤维支架。

心血管疾病是当今社会的医疗保健问题。临床上可用的人工合成血管移植物具有血栓形成作用，并可引起内膜增生。快速内皮化和匹配的机械性能是设计功能性血管移植物时要考虑的两个主要要求。在此，将电纺制的管状纤维（eTF）支架在腔表面用原弹性蛋白生物功能化。通过ζ电势的增加和NH ₂基团的插入证实了腔表面功能化。Tropoelastin通过其-NH ₂固定化分别在活化的或氨基水解的eTF支架上的-COOH或-COOH基团，以研究暴露的官能团对人内皮细胞（EC）行为的影响。拉伸性能表明，功能化的eTF支架的强度和刚度在天然血管的范围内。固定在活化eTF支架上的Tropoelastin促进了更高的代谢活性和EC的增殖，而固定在氨基化eTF支架上时，观察到了更高的蛋白质合成。这些具有生物功能的eTF支架是有前途的小直径血管移植物，可促进快速内皮化并具有相容的机械性能。

更新日期：2020-09-14

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