Abstract

Vasopressors are frequently given in hemodynamically unstable patients with severe Candida sepsis. While catecholamines can aggravate sepsis-induced immune dysfunction and modulate bacterial virulence traits, their impact on fungal pathogenicity is poorly understood. Using IncuCyte time-lapse microscopy and a fruit fly candidiasis model, we studied growth rates, morphogenesis, stress tolerance, and virulence of C. albicans cocultured with epinephrine and norepinephrine. We found that pharmacologically attainable catecholamine serum concentrations caused minimal changes to in vitro growth kinetics, filamentation, and fungal resistance to thermal or oxidative stress. Similarly, exposure of C. albicans to catecholamines did not alter the survival of infected flies.

中文翻译：

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肾上腺素和去甲肾上腺素的药理血清浓度不影响白色念珠菌的生长速度，形态发生，胁迫耐受性和毒力。

摘要

患有严重念珠菌败血症的血液动力学不稳定的患者经常使用血管加压药。儿茶酚胺可加重败血症诱导的免疫功能障碍并调节细菌毒力特征，但对真菌致病性的影响知之甚少。使用IncuCyte延时显微镜和果蝇念珠菌病模型，我们研究了与肾上腺素和去甲肾上腺素共培养的白色念珠菌的生长速率，形态发生，胁迫耐受性和毒力。我们发现，药理学上可达到的儿茶酚胺血清浓度对体外生长动力学，丝化作用以及对热或氧化应激的真菌抗性影响不大。同样，白色念珠菌的暴露 儿茶酚胺并没有改变被感染果蝇的存活率。