Abstract

Mucin-type O-glycosylation is one of the most common posttranslational modifications of proteins. The abnormal expression of various polypeptide GalNAc-transferases (GalNAc-Ts) which initiate and define sites of O-glycosylation are linked to many cancers and other diseases. Current O-glycosyation prediction programs utilize O-glycoproteomics data obtained without regard to the transferase isoform (s) responsible for the glycosylation. With 20 different GalNAc-Ts in humans, having an ability to predict and interpret O-glycosylation sites in terms of specific GalNAc-T isoforms is invaluable.To fill this gap, ISOGlyP (Isoform-Specific O-Glycosylation Prediction) has been developed. Using position-specific enhancement values generated based on GalNAc-T isoform-specific amino acid preferences, ISOGlyP predicts the propensity that a site would be glycosylated by a specific transferase. ISOGlyP gave an overall prediction accuracy of 70% against in vivo data, which is comparable to that of the NetOGlyc4.0 predictor. Additionally, ISOGlyP can identify the known effects of long- and short-range prior glycosylation and can generate potential peptide sequences selectively glycosylated by specific isoforms.ISOGlyP is freely available for use at ISOGlyP.utep.edu. The code is also available on GitHub (https://github.com/jonmohl/ISOGlyP).

中文翻译：

---

ISOGlyP：同种型特异性粘蛋白型 O-糖基化的从头预测

摘要

粘蛋白型O-糖基化是蛋白质最常见的翻译后修饰之一。启动和确定 O-糖基化位点的各种多肽 GalNAc-转移酶 (GalNAc-Ts) 的异常表达与许多癌症和其他疾病有关。当前的 O-糖基化预测程序利用获得的 O-糖蛋白质组学数据，而不考虑负责糖基化的转移酶异构体。人类有 20 种不同的 GalNAc-T，能够根据特定的 GalNAc-T 异构体预测和解释 O-糖基化位点的能力是非常宝贵的。为了填补这一空白，开发了 ISOGlyP（异构体特异性 O-糖基化预测）。使用基于 GalNAc-T 异构体特异性氨基酸偏好生成的位置特异性增强值，ISOGlyP 预测位点被特定转移酶糖基化的倾向。ISOGlyP 对体内数据的总体预测准确度为 70%，与 NetOGlyc4.0 预测器的预测准确度相当。此外，ISOGlyP 可以识别已知的长程和短程糖基化效应，并可以生成被特定异构体选择性糖基化的潜在肽序列。ISOGlyP 可在 ISOGlyP.utep.edu 上免费使用。该代码也可在 GitHub (https://github.com/jonmohl/ISOGlyP) 上获得。ISOGlyP 可以识别已知的长程和短程先前糖基化的影响，并且可以生成被特定异构体选择性糖基化的潜在肽序列。ISOGlyP 可在 ISOGlyP.utep.edu 上免费使用。该代码也可在 GitHub (https://github.com/jonmohl/ISOGlyP) 上获得。ISOGlyP 可以识别已知的长程和短程先前糖基化的影响，并可以生成被特定异构体选择性糖基化的潜在肽序列。ISOGlyP 可在 ISOGlyP.utep.edu 免费使用。该代码也可在 GitHub (https://github.com/jonmohl/ISOGlyP) 上获得。