Abstract In this study, different concentrations (0, 0.025, 0.05, 0.075, 0.1 and 0.2 mM) of iron nanoparticles (FeNPs) were used to replace 0.1 mM Fe-EDTA in half-strength MS medium to assess their effect on root growth, antioxidant enzyme activity and mineral absorbability of carnation plantlets cultured in in vitro system (MO) and microponic system (MP). The results showed that MP supplemented with 0.075 mM FeNPs was the most optimal culture condition for not only rooting stage, but also antioxidant activities and mineral absorption of carnation. The 3-week-old shoots cultured in this condition obtained the highest growth values with plant height (6.67 cm), root number (9.00 roots/plantlet), average root length (1.80 cm), fresh weight (70.33 mg), dry weight (5.67 mg), dry mass ratio (8.05 %), chlorophyll a (1.96 mg g−1 FW) and chlorophyll b (0.87 mg g−1 FW). Furthermore, the antioxidant activity analysis results showed that the activity of superoxide dismutase (28.52 U g-1 prot), catalase (540.98 U g-1 prot), and ascorbate peroxidase (0.55 U g-1 prot) in plantlets grown in MP system supplemented with 0.075 mM FeNPs were higher than those in MO system (14.15, 426.66 and 0.46 U g-1 prot; respectively) and also in the control (7.84, 309.08, 0.35 U g-1 prot; respectively). In addition, the absorption of potassium (205.51 mg g−1 DW), calcium (27.70 mg g−1 DW), magnesium (25.55 mg g−1 DW) and the iron-uptake rate (79.37 %) were highest in the MP system added with 0.075 mM FeNPs, as compared with those of the others. Plantlets derived from culture in MP system supplemented with FeNPs gave higher survival rate, better growth and development than that of the control treatment (used 0.1 mM Fe-EDTA). The plantlets were investigated after six weeks transplanting into the greenhouse.

中文翻译：

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评估在两种添加铁纳米颗粒的培养系统中培养的康乃馨（石竹“Express golem”）植株的根生长、抗氧化酶活性和矿物质吸收能力

摘要 在本研究中，使用不同浓度（0、0.025、0.05、0.075、0.1 和 0.2 mM）的铁纳米粒子 (FeNPs) 代替半强度 MS 培养基中的 0.1 mM Fe-EDTA 来评估它们对根生长的影响，在体外系统 (MO) 和微耕系统 (MP) 中培养的康乃馨植株的抗氧化酶活性和矿物质吸收能力。结果表明，添加0.075 mM FeNPs的MP不仅是康乃馨生根期、抗氧化活性和矿物质吸收的最佳培养条件。在此条件下培养的 3 周龄芽获得最高的生长值，包括株高 (6.67 cm)、根数 (9.00 根/小植株)、平均根长 (1.80 cm)、鲜重 (70.33 mg)、干重(5.67 mg)、干重比 (8.05 %)、叶绿素 a (1.96 mg g-1 FW) 和叶绿素 b (0. 87 毫克 g-1 FW)。此外，抗氧化活性分析结果表明，MP系统中生长的植株中超氧化物歧化酶（28.52 U g-1 prot）、过氧化氢酶（540.98 U g-1 prot）和抗坏血酸过氧化物酶（0.55 U g-1 prot）的活性补充 0.075 mM FeNPs 的含量高于 MO 系统（分别为 14.15、426.66 和 0.46 U g-1 prot）和对照（分别为 7.84、309.08、0.35 U g-1 prot）。此外，钾（205.51 mg g-1 DW）、钙（27.70 mg g-1 DW）、镁（25.55 mg g-1 DW）和铁吸收率（79.37 %）在MP中的吸收最高与其他系统相比，系统添加了 0.075 mM FeNPs。在补充有 FeNPs 的 MP 系统中培养的幼苗比对照处理（使用 0. 1 mM Fe-EDTA）。在移植到温室中六周后调查小植株。