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A phenylpropanoid diglyceride associates with the leaf rust resistance Lr34res gene in wheat
Phytochemistry ( IF 3.2 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.phytochem.2020.112456 Nandhakishore Rajagopalan 1 , Yuping Lu 1 , Ian W Burton 2 , Fanny Monteil-Rivera 3 , Annamaria Halasz 4 , Elsa Reimer 5 , Rebecca Tweidt 6 , Anita Brûlé-Babel 7 , Hadley R Kutcher 6 , Frank M You 8 , Sylvie Cloutier 8 , Miroslava Cuperlovic-Culf 9 , Colin W Hiebert 5 , Brent D McCallum 5 , Michele C Loewen 10
Phytochemistry ( IF 3.2 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.phytochem.2020.112456 Nandhakishore Rajagopalan 1 , Yuping Lu 1 , Ian W Burton 2 , Fanny Monteil-Rivera 3 , Annamaria Halasz 4 , Elsa Reimer 5 , Rebecca Tweidt 6 , Anita Brûlé-Babel 7 , Hadley R Kutcher 6 , Frank M You 8 , Sylvie Cloutier 8 , Miroslava Cuperlovic-Culf 9 , Colin W Hiebert 5 , Brent D McCallum 5 , Michele C Loewen 10
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The gene Lr34res is one of the most long-lasting sources of quantitative fungal resistance in wheat. It is shown to be effective against leaf, stem, and stripe rusts, as well as powdery mildew and spot blotch. Recent biochemical characterizations of the encoded ABC transporter have outlined a number of allocrites, including phospholipids and abscisic acid, consistent with the established general promiscuity of ABC transporters, but ultimately leaving its mechanism of rust resistance unclear. Working with flag leaves of Triticum aestivum L. variety 'Thatcher' (Tc) and a near-isogenic line of 'Thatcher' into which the Lr34res allele was introgressed (Tc+Lr34res; RL6058), a comparative semi-targeted metabolomics analysis of flavonoid-rich extracts revealed virtually identical profiles with the exception of one metabolite accumulating in Tc+Lr34res, which was not present at comparable levels in Tc. Structural characterization of the purified metabolite revealed a phenylpropanoid diglyceride structure, 1-O-p-coumaroyl-3-O-feruloylglycerol (CFG). Additional profiling of CFG across a collection of near-isogenic lines and representative Lr34 haplotypes highlighted a broad association between the presence of Lr34res and elevated accumulations of CFG. Depletion of CFG upon infection, juxtaposed to its relatively lower anti-fungal activity, suggests CFG may serve as a storage form of the more potent anti-microbial hydroxycinnamic acids that are accessed during defense responses. Altogether these findings suggest a role for the encoded LR34res ABC transporter in modifying the accumulation of CFG, leading to increased accumulation of anti-fungal metabolites, essentially priming the wheat plant for defense.
中文翻译：
苯丙酸甘油二酯与小麦叶锈病抗性 Lr34res 基因相关
Lr34res 基因是小麦定量真菌抗性最持久的来源之一。它被证明对叶锈病、茎锈病和条锈病以及白粉病和斑点病有效。编码的 ABC 转运蛋白的最新生化特征概述了许多同种异质体，包括磷脂和脱落酸，与公认的 ABC 转运蛋白的普遍混杂一致，但最终使其抗锈病机制尚不清楚。使用小麦品种“撒切尔”（Tc）的旗叶和 Lr34res 等位基因渗入的“撒切尔”近等基因系（Tc+Lr34res；RL6058），对富含类黄酮的提取物进行的比较半靶向代谢组学分析显示，除了一种代谢物在 Tc+Lr34res 中积累外，几乎完全相同的特征，其在 Tc 中的含量不存在可比水平。纯化代谢物的结构表征揭示了苯丙酸甘油二酯结构，1-Op-香豆酰-3-O-阿魏酰甘油（CFG）。对一组近等基因系和代表性 Lr34 单倍型的 CFG 进行额外分析，突出了 Lr34res 的存在与 CFG 积累增加之间的广泛关联。感染后 CFG 的消耗，与其相对较低的抗真菌活性并列，表明 CFG 可以作为更有效的抗微生物羟基肉桂酸的储存形式，在防御反应期间使用。
更新日期：2020-10-01
中文翻译：
苯丙酸甘油二酯与小麦叶锈病抗性 Lr34res 基因相关
Lr34res 基因是小麦定量真菌抗性最持久的来源之一。它被证明对叶锈病、茎锈病和条锈病以及白粉病和斑点病有效。编码的 ABC 转运蛋白的最新生化特征概述了许多同种异质体，包括磷脂和脱落酸，与公认的 ABC 转运蛋白的普遍混杂一致，但最终使其抗锈病机制尚不清楚。使用小麦品种“撒切尔”（Tc）的旗叶和 Lr34res 等位基因渗入的“撒切尔”近等基因系（Tc+Lr34res；RL6058），对富含类黄酮的提取物进行的比较半靶向代谢组学分析显示，除了一种代谢物在 Tc+Lr34res 中积累外，几乎完全相同的特征，其在 Tc 中的含量不存在可比水平。纯化代谢物的结构表征揭示了苯丙酸甘油二酯结构，1-Op-香豆酰-3-O-阿魏酰甘油（CFG）。对一组近等基因系和代表性 Lr34 单倍型的 CFG 进行额外分析，突出了 Lr34res 的存在与 CFG 积累增加之间的广泛关联。感染后 CFG 的消耗，与其相对较低的抗真菌活性并列，表明 CFG 可以作为更有效的抗微生物羟基肉桂酸的储存形式，在防御反应期间使用。
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