Purpose

Decreased nitric oxide bioavailability in skin contributes to impaired wound healing in type 2 diabetes (T2D). This study aims at determining effects of acidified nitrite on wound closure as well as inflammatory and antioxidants markers in wound tissue of rats with T2D.

Main methods

Skin wound was made on the back of rats 28 days after the induction of T2D (high-fat diet/low-dose of streptozotocin). Control and diabetic rats were subdivided into two subgroups: Untreated control (C), acidified nitrite-treated control (CN), untreated diabetes (D), and acidified nitrite-treated diabetes (DN). Acidified nitrite was applied once daily from day 3 to day 28 and the wounds were photographed for macroscopic changes. On days 3, 7, 14, 21, and 28 after wounding, wound levels of inflammatory and antioxidant markers were measured.

Results

Half closure time (CT50%) was significantly lower in acidified nitrite-treated diabetic rats compared to untreated ones (5.1 vs. 8.0 days, P < 0.001). Inflammatory response was delayed in diabetic rats and persistent inflammatory response was observed at day 14 after wounding. Acidified nitrite application restored the inflammatory response and antioxidant levels to control values.

Conclusions

Acidified nitrite accelerated wound healing in rats with T2D by restoring delayed inflammatory response and augmentation of antioxidant defense.

中文翻译：

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酸化的亚硝酸盐改善2型糖尿病大鼠的伤口愈合：氧化应激和炎症的作用。

目的

皮肤中一氧化氮的生物利用度降低会导致2型糖尿病（T2D）伤口愈合受损。这项研究旨在确定酸化的亚硝酸盐对T2D大鼠伤口闭合以及伤口组织中炎性和抗氧化剂标记的影响。

主要方法

诱导T2D（高脂饮食/低剂量链脲佐菌素）后28天，在大鼠背部制作皮肤伤口。将对照组和糖尿病大鼠分为两个亚组：未治疗的对照组（C），酸化的亚硝酸盐治疗的对照组（CN），未治疗的糖尿病（D）和酸化的亚硝酸盐治疗的糖尿病（DN）。从第3天到第28天，每天使用一次酸化的亚硝酸盐，并为伤口拍照以观察宏观变化。受伤后第3、7、14、21和28天，测量伤口的炎症和抗氧化剂标记水平。

结果

与未治疗的大鼠相比，酸化的亚硝酸盐治疗的糖尿病大鼠的半关闭时间（CT50％）显着降低（5.1天与8.0天，P <0.001）。糖尿病大鼠的炎症反应被延迟，并且在受伤后第14天观察到持续的炎症反应。酸化亚硝酸盐的应用使炎症反应和抗氧化剂水平恢复到控制值。

结论

酸化的亚硝酸盐通过恢复延迟的炎症反应和增强抗氧化防御能力，加速了T2D大鼠的伤口愈合。